Quinolones assays

Snitkoff et al. [75] reported the development of an EIA for the detection of ciprofloxacin in serum, which was sensitive at picogram per milliliter levels of the antibiotic and no cross-reaction with its metabolites was observed. Gobbo et al. [118] recently described the production of PAb for ciprofloxacin with the aim of detecting fluoroquinolones in Brazilian livestock. On the other hand, Bucknall et al. [77] produced antibodies for quinolones and fluoroquinolones with the aim of developing both generic and specific immunoassays. ELISAs for ciprofloxacin, enrofloxacin, flumequine, and nalidixic acid were developed with sensitivity values around 4 pg kg 1 (on both the generic and specific assays) in bovine milk and ovine kidney. 

Huet, A.C., C. Charlier, S.A. Tittlemier, et al. 2006. Simultaneous determination of (fluoro)quinolone antibiotics in kidney, marine products, eggs, and muscle by enzyme-linked immunosorbent assay (ELISA). J. Agric. Food Chem. 54 2822-2827. 

The first step in this direction is to correlate the permeability values obtained in the Caco-2 cell lines (apical-basolateral direction, Pay) with the fraction of dose absorbed in vivo in rat For this proposal eight fluoroquinolones were assayed and the results found with Caco-2 cell lines were compared with those obtained in vivo in rat In the Caco-2 cell lines the permeability of the quinolones was evaluated at different initial concentration, in order to test for no linearities in the absorption process. For some of them, it was observed that a secretion system worked in the opposite direction to passive diffusion for this reason the permeability value used for the correlations, in the case of secretion, was the one obtained at the highest concentration of the quinolone, which corresponds to saturation of the secretion process. 

Griggs, D.J. Wise, R. A simple isocratic high pressure hquid chromatographic assay of quinolones in serum. J. Antimicroh. Chemother. 1989, 24, 437-445. 

Takacs-Novak et al. (14) used a potentiometric assay in combination with UV spectroscopy and NMR to study the acid-base properties and protonation equilibria for lomefloxacin and other quinolone antibacterials. Okabayashi et al. used potentiometric measurements to determine the stability constants of metal ions with several quinolones including lomefloxacin (20). 

Bogialli S, D Ascenzo G, Di Corcia A, Lagana A, Nicolardi S, A simple and rapid assay based on hot water extraction and liquid chromatography-tandem mass spectrometry for monitoring quinolone residues in bovine miUc, Food Chem. 2008 108 354-360. 

Huet AC, Charlier C, Singh G, et ah, Development of an optical surface plasmon resonance biosensor assay for (fluoro)quinolones in egg, fish, and poultry meat, Anal. Chim. Acta 2008 623(2) 195-203. 

Cyclic pentapeptide is a potent inhibitor of a4] l-mediated cell adhesion to CS-1 site and VCAM with IC50 ranging from 2 to 9 pM in cell adhesion assays. Under this background, Ho and Broka reported the synthesis of peptidomimetic tricyclic tetrahydrobenzo [ij] quinolone in 2000 [95]. Palladium-catalyzed alkoxycarb-onylation of aryl triflate was applied and produced the needed intermediate in moderate yield (Scheme 10.20). 

In contrast to the above results, the basophil activation test was positive to the implicated quinolone in all five subjects with immediate reactions to a quinolone who were tested [19 ]. However, this assay quantified CD203C, which may be a more sensitive marker than CD63 for detecting IgE-mediated allergy. 

Keywords histidine assay, singlet oxygen, anti-bacterial, photochemical activity, quinolone, reactive oxygen species... 

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