Quantitative autoradiography

The difference in rates of release of BCNU from wafers produced by the trituration or solution methods is also seen in vivo (11,14), as is shown in Fig. 6. Wafers of PCPP-SA 20 80 were prepared by either the solution or trituration methods, as described above, and were implanted into the brains of rabbits. The animals were sacrificed at various times after implantation and the brains were removed, fixed, and processed for quantitative autoradiography. To quantitate the percentage of the brain exposed to BCNU released from these wafers, the following calculation was performed. The percentage of the brain in which the radioactivity from the tritiated BCNU released from the wafers exceeded the background counts by at least two standard deviation units was plotted as a function of time following implantation in Fig. 6. A control set of rabbits had a solution of BCNU injected directly into the same location in the... 

FIGURE 6 Distribution of [ HlBCNU-associated radioactivity time in rabbit brain. The distribution of radioactivity was measured using quantitative autoradiography techniques following implantation of discs described for Fig. 5 into the brains of rabbits. Details of the experimental procedure are described in the text. 

Hensler, J. G., Ferry, R. C., Labow, D. M., Kovachich, G. B. 8r Frazer, A. (1994). Quantitative autoradiography of the serotonin transporter to assess the distribution of serotonergic projections from the dorsal raphe nucleus. Synapse 17, 115. 

Kilpatrick, G. J., Jones, B. J. Tyers, M. B. (1988). The distribution and specific binding of the 5-HT3 receptor ligand [3H] GR65630 in rat brain using quantitative autoradiography. Neurosci. Lett. 94, 156-60. 

Adenosine A-l receptor density was examined in rat brain by means of quantitative autoradiography to obtain a detailed anatomical overview of the changes during ageing (Murillo-Rodriguez et al. 2004). A-l receptor binding was assessed in young, old, and senescent animals of 3, 24, and 30 months old, respectively. 

Brunswick, D.J., Benmansour, S., Tejani-Butt, S.M., Hauptmann, M. Effects of high-dose methamphetamine on monoamine uptake sites in rat brain measured by quantitative autoradiography. Synapse. 11 287, 1992. 

Withdraw the slide immediately or wait a few seconds to derive a thicker emulsion layer (DO NOT dip each slide repeatedly to achieve a thicker emulsion instead, reduce withdrawal rate or emulsion temperature). Consult Rogers (7) for selecting an appropriate emulsion thickness (1.5-2.0 pm but depends on emulsion and isotope in question), especially if one is contemplating quantitative autoradiography. Move slide away from the emulsion so that excess emulsion does not drip back onto the surface of the emulsion creating bubbles. 

Domer P. Photometric methods in quantitative autoradiography, in Microautoradiography and Electron Probe Analysis. Their Application to Plant Physiology (Luttge U, ed.), Springer Verlag, New York, 1972, pp. 7M8. 

Administration of Radioisotopes, Tissue Preparation, and Emulsions Resolution and Quantitative Autoradiography Limitations of EM Autoradiographs Protocols Conclusions References... 

J. T. Greenamyre, D.S. Higgins, R.V. Ellers, Quantitative autoradiography of dihydror-otenone binding to complex I of the electron transport chain, J. Neurochem. 59 (1992) 746-749. 

Ryu, J.H., Yanai, K., Sakurai, E., Kim, C.Y. and Watanabe, T. (1995). Ontogenetic development of histamine receptor subtypes in rat brain demonstrated by quantitative autoradiography. Developmental Brain Res. 87, 101-110. 

Fabin, B., andE. Touitou. 1991. Localization of lipophilic molecules penetrating rat skin in vivo by quantitative autoradiography. Int J Pharm 74 59. 

Verge D, Daval G, Marcinkiewicz M, et al. Quantitative autoradiography of multiple 5-HT1 receptor subtypes in the brain of control or 5,7-dihydroxytryptamine-treated rats. J Neurosci 1986 6 3474-3482. 

Fischette CT, Nock B, Renner K. Effects of 5,7-dihydroxytryptamine on sero-toninl and serotonin2 receptors throughout the rat central nervous system using quantitative autoradiography. Brain Res 1987 421 263-279. 

Barone P, Jordan D, Atger F, Kopp N, Fillion G. Quantitative autoradiography of 5-HT1D and 5-HT1E binding sites labelled by [3H]5-HT, in frontal cortex and the hippocampal region of the human brain. Brain Res 1994 638 85-94. 

Knable MB, Hyde TM, Herman MM, Carter JM, Bigelow L, et al. 1994. Quantitative autoradiography of dopamine-Dl receptors, D2 receptors, and dopamine uptake sites in postmortem striatal specimens from schizophrenic patients. Biol Psychiatry 36 827-835. 

In the amygdaloid complex, D2 receptors, evaluated by quantitative autoradiography, showed low density in most nuclei, but a relatively high concentration was detected in the central nucleus, with a medial to lateral gradient (Bouthenet et al., 1987 Scibilia et al., 1992). 

Filloux FM, Wamsley JK, Dawson TM (1987a) Dopamine D-2 auto- and postsynaptic receptors in the nigrostriatal system of the rat brain localization by quantitative autoradiography with [3H]sulpiride. Eur J Pharmacol 735 61-68. 

Jastrow TR, Richfield E, Gnegy ME (1984) Quantitative autoradiography of [3H]sulpiride binding sites in rat brain. Neurosci Lett 57 47-53. 

Morelli M, Mennini T, Di Chiara G (1988) Nigral dopamine autoreceptors are exclusively of the D2 type quantitative autoradiography of [125I]iodosulpride and [125I]SCH 23982 in adjacent brain sections. Neuroscience 27 865-870. 

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