PVIII

Bichel Bomb. It is briefly described in Vol 1, pVIII of this Encyclopedia. As supplement to this are illustrations from the books of Colver (1918), p606 and Vivas, Feiganspan Ladreda, Vol 4 (1945). P.101... 

To achieve surface display, five of the Ml3 coat proteins have been used in fusion to foreign protein fragments. In the most widespread system the antibody is coupled to the N-terminus or second domain of the minor coat protein pill (11,12,14). The naive function of the three to five copies of the pill, in particular their N-terminal domain, is to provide binding of the phage to the f-pili of E. coli to initiate infection (39). The major coat protein (pVIII) has been used as an alternative fusion partner, with only very few successes reported in the past decade (40). This fusion technique is more useful for the display of short peptides (41,42). Fusions to pVI have been tried, but not yet with antibodies (43). pVII and pIX were... 

For vector nomenclature, see Table 1. Table 2 includes and extends the data compiled by Clackson and Wells [5], Hybrid protein1 designates the presented ligand fused to a coat protein (minor coat proteins, normally five copies of each of the wild-type proteins or the major coat protein (pVIII) normally present at ca. 2700 copies or more if the vector is larger). 

FIGURE 16.4 Schematic representation of phage (b) and phagemid (c) display of polypeptides fused to pill and pVIII coat proteins, compared to wild-type phage (a). 

The one-gene system display (phage) is a good system for the display of small peptides, which do not alter the structure of the coat protein and do not prevent its assembly into viral particles. For the display of larger peptides the two-gene system (phagemid) is preferred or sometimes required, as for pVIII fusion. 

Several types of Kistler pressure transducers and adapters are used at Picatinny Arsenal (See also Bichel Bomb Bichel Pressure Gage in Vol 1, pVIII of this Encycl and Chamber Pressure in Vol 2, pC147-R)... 

Figure 19-26. Sequence and sequence coverage of pVIII (matched sequences are highlighted in boldface). Oxidation of Met ° was detected by peptide mapping. (Reprinted from reference 138, with permission of Elsevier Science B.V.)...

The presence of pVIII in empty capsid confirms that the viral particle is at an early stage of virus assembly. Based on this finding and the uniqueness of pVIII to the empty capsid of the adenovirus, an assay was developed to quantify the empty capsid contaminants by measuring the amount of pVIII detected in SDS-PAGE during the recombinant adenovirus preparation [136]. 

Inoviridae—6.4-kb ssDNA genome is enclosed in a flexible helical filament of 2800 copies of the major coat protein pVIII. Proteins in smaller copy number cap the ends... 

Fold Coat protein pVIII, single a helix packed in a helical array ssDNA-binding protein, antiparallel f3 sheet... 

Huse WD, Stinchcombe TJ, Glaser SM, Starr L, MacLean M, Hellstrom KE, Hellstrom I, Yelton DE. Application of a filamentous phage pVIII fusion protein system suitable for efficient production, screening, and mutagenesis of F(ab) antibody fragments. J. Immunol. 1992 149(12) 3914-3920. 

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