SsDNA-binding protein

C2HC Zn finger (smart00343) KCYNCGKPGHIARDC PS Found in the Nucleocapsid protein of retrovirus. Also found in eukaryotic RNA-or ssDNA-binding proteins Human Rb-BP 6 (NP 008841)-retmoblatoma-binding protein 6 378, 379... 

VirE2 ssDNA-binding protein that coats the T-strand part of the T-DNA... 

The construction of intact virus particles from the genomic DNA and protein subunits is often a complex process. It is simplest for the small filamentous ssDNA viruses (Fig. 7-7). The subunits are synthesized as soluble proteins, which enter the cell membrane, then lose their leader sequences. As the viral DNA coated by the viral gene 5 ssDNA-binding protein enters the membrane, the binding protein is replaced by the coat subunits, 422-424... 

Fold Coat protein pVIII, single a helix packed in a helical array ssDNA-binding protein, antiparallel f3 sheet... 

Cotiphage strains M13, fd, Pfl, Xf ssDNA-binding protein pV (Skinner et at, 1994), m or coat protein pVlII (Marvin et at, 1994 McDonnell et at, 1993 Nambudripad et at, 1991a)... 

Fiber shaft plus head domain. Shaft is a novel triple /l-spiral fibrous fold. Head is an antiparallel f) barrel with a novel Greek key-based strand topology ssDNA-binding protein unique fold that is mosdy a, with some fi... 

DNAs are constantly subjected to denaturing forces in vivo due to physical interactions with various dsDNA-binding or destabilizing proteins and ssDNA-binding proteins. The local reversible unwinding (or melting) is indeed of central biological importance because it is the primary step in DNA replication performed by DNA polymerases (Chapter 6) and in transcription performed by RNA polymerases (Chapter 7). 

RNase, as a ssDNA-binding protein, does not affect the initial rate but gives a modest increase in the ligation yield. The presence of RNase A enables the ligation between duplex forms of oligodeoxynucleotides which are not substrates under normal conditions (8). 

The ligation between duplex forms of oligodeoxynucleotides is not catalyzed by RNA ligase under normal conditions. However, the reaction can occur in the presence of ssDNA-binding protein, e.g., RNase A (8). Remarkably, the blunt-end DNA fragments (—GG/CC—) generated by Haelll restriction enzyme digestion are active donors (22). 

Specificity alterations in presence of T4 ssDNA-binding protein. In the... 

V. Competition of exonuclease and polymerase activities. The exonuclease activity of T4 DNA Pol on the ssDNA substrate is not affected by the addition of dNTPs. However, it is inhibited by the presence of the ssDNA-binding protein gp32. When dsDNA is used as a substrate, the addition of complementary dNTPs causes a reduction in the rate of hydrolysis due to the emergence of competing polymerase activity. 

With poly(dAT) as the template-primer, the error rate is l/2500 the fidelity increases up to fivefold when the . coli ssDNA-binding protein is added to the reaction (55). 

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