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PVA oxidase

Poly(vinyl alcohol) oxidase PVA oxidase, poly(vinyl alcohol) oxidoreductase, poly(vinyl alcohol) dehydrogenase ... [Pg.159]

Secondary-alcohol oxidoreductase Polyvinyl alcohol oxidase, PVA oxidase, SAO —... [Pg.159]

Another basic issue to be considered was represented by the influence of PHB on the biodegradability of PVA in the presence of specific PVA-degrading microorganims, by considering that biodegradation of the polyester and of the polyhydroxylated polymer is strictly mediated by PHB-depolymerase and PVA-oxidase specific enzymes, respectively. In this connection, the biodegradability of both a graft copolymer and a cast blend was ascertained in the presence of a select bacterial culture able to utilize PVA. [Pg.338]

A flow injection optical fibre biosensor for choline was also developed55. Choline oxidase (ChOX) was immobilized by physical entrapment in a photo-cross-linkable poly(vinyl alcohol) polymer (PVA-SbQ) after adsorption on weak anion-exchanger beads (DEAE-Sepharose). In this way, the sensing layer was directly created at the surface of the working glassy carbon electrode. The optimization of the reaction conditions and of the physicochemical parameters influencing the FIA biosensor response allows the measurement of choline concentration with a detection limit of 10 pmol. The DEAE-based system also exhibited a good operational stability since 160 repeated measurements of 3 nmol of choline could be performed with a variation coefficient of 4.5%. [Pg.171]

The blend of PVA with PEG- modified glucose oxidase could be used as glucose sensor characterized by the linearity of calibration curve in the range of concentration by 5 x 10"5 - 5 x 10"3 mol glucose L 1 [194],... [Pg.155]

Leca and Marty [26] Cider Wine Whisky Co-immobilisation of alcohol dehydrogenase (ADH), NADH oxidase and high molecular weight NAD (NAD-dextran) in a poly(vinyl alcohol) bearing stryrylpyridinium groups (PVA-SbQ) matrix Pt/+550 vs. Pt ... [Pg.266]

All potentials vs. screen-printed pseudo Ag/AgCl reference, except values marked with double-dagger (J), which are vs. screen-printed Ag/Pd, and 02 those marked with asterisk ( ), which are vs. Ag/3M AgCl double-junction reference electrode. Abbreviations LDH lactate dehydrogenase, LOD lactate oxidase, PVA-SbQ styrlpyridinium-modified poly(vinyl alcohol), FIA flow injection analysis. aLinear ranges using a dialysis system. [Pg.507]

Acetylcholineesterase and choline oxidase Prepared by mounting a carbon fiber (200 pm diam) in a glass capillary with silver paste and epoxy resin, electrochemically pretreating the electrode from 0 to 1.2 V for 15 min, and dipping the electrode in 11% PVA-Styryl pyridinium solution containing AChE and ChO. The calibration graph was rectilinear from 0.2 to 1 mM of ACh. The response time was 0.8 min. [76]... [Pg.34]

Acetylcholineesterase and choline oxidase Enzyme membrane in H20 was treated with 11% solution of PVA-SbQ (polyvinyl alcohol) with styryl pyridinium groups. Mixture was spread on a cellulose nitrate membrane and air dried. The membrane was exposed to UV radiation for 3 h and stored at 4°C. The enzyme membrane was fixed with a Pt electrode. Sample was dissolved in phosphate buffer and measured. The best results were obtained at pH 8 and at 30°. The calibration graph was rectilinear for 5mM ACh. The storage stability of the dry membrane was excellent. [77]... [Pg.34]

Doretti, L. Ferrara, D. Gattolin, P. Lora, S. Schiavon, F. Veronese, F.M. PEG-modified glucose oxidase immobilized on a PVA cryogel membrane for amperometric biosensor applications. Talanta 1998, 45, 891-898. [Pg.2038]

In the case of PVA, which is one of the few carbon-chain polymers that does not need to be abiotically peroxidised before biological attack, polyketones are the initial products and these are catabolysed to carboxylic acids and bioassimilated by bacteria e.g. Pseudomonas). Kawai has shown (personal communication) that the PEGs behave rather similarly and are bioassimilated by Sphingomonas. The active enzymes are believed to be PEG dehydrogenase coupled with cytochrome c, the oxidase enzyme of the baeterial respiratory system. Since the polyethers are also very peroxidisable abiotically, abiotic peroxidation may also play a part in the overall process. [Pg.101]

A variety of polymeric films were produced by this method these include poly(2-hydroxyethyl methacrylate) that have been applied in GOx-based sen-sor, PVA in glucose oxidase, and lactate oxidase immobilization, and... [Pg.316]

Doretti L, Ferrara D, Gattolin P, Lora S, Schiavon F, Veronese FM (1998) PEG-modified glucose oxidase immobilized on PVA cryogel membrane for amperomefric biosensor applications. Talanta 45 891-898... [Pg.276]

Figure 7.12 Proposal pathway for PVA degradation by various enyme systems. 1) Pyrroloquinoline quinine (PQQ)-dependent PVA hydrogenase 2) oxidised PVA hydrolase 3) PVA-degrading enzyme with dehydrogenase and aldolase activities 4) secondary alcohol oxidase 5) p-diketone hydrolase and 6) PVA-degrading enzyme with oxidase and hydrolase activity. Reproduced with permission from M. Shimao, Current Opinion in Biotechnology, 2001, 12, 3, 242. Figure 7.12 Proposal pathway for PVA degradation by various enyme systems. 1) Pyrroloquinoline quinine (PQQ)-dependent PVA hydrogenase 2) oxidised PVA hydrolase 3) PVA-degrading enzyme with dehydrogenase and aldolase activities 4) secondary alcohol oxidase 5) p-diketone hydrolase and 6) PVA-degrading enzyme with oxidase and hydrolase activity. Reproduced with permission from M. Shimao, Current Opinion in Biotechnology, 2001, 12, 3, 242.
Glucose oxidase + galactose oxidase + lactate oxidase + peroxidase IPN PVA-Fn5-Si02 composite Glucose, galactose, lactose, lactate OBS 305... [Pg.757]

Acetylcholine esterase + choline oxidase IPN PVA-Pd-C-FnS-SiOz Acetylcholine, choline EBS 305... [Pg.757]

See other pages where PVA oxidase is mentioned: [Pg.160]    [Pg.161]    [Pg.161]    [Pg.432]    [Pg.377]    [Pg.378]    [Pg.160]    [Pg.161]    [Pg.161]    [Pg.432]    [Pg.377]    [Pg.378]    [Pg.482]    [Pg.59]    [Pg.153]    [Pg.160]    [Pg.482]    [Pg.110]    [Pg.2030]    [Pg.2030]    [Pg.137]    [Pg.353]    [Pg.80]    [Pg.36]    [Pg.36]    [Pg.151]    [Pg.180]    [Pg.181]    [Pg.487]    [Pg.153]    [Pg.286]    [Pg.136]   
See also in sourсe #XX -- [ Pg.160 ]



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