Purkinje cells nerve growth factor

Nerve growth factor and nerve growth factor-receptor protein in Purkinje cells... 

Nerve growth factor-like immunoreactivity was present in Purkinje cell somata and dendrites, with dense labelling in the paraflocculus, and in neurons of the cerebellar nuclei and the lateral vestibular nucleus of rat cerebellum (Nishio et ah, 1994). All but a few of the Purkinje cells of the adult rat cerebellum stain with an antiserum against basic fibroblast growth factor. Staining was observed in all cellular compartments (Matsuda et ah, 1992). 

P75 nerve growth factor-receptor protein (NGF-R) is present in developing and adult Purkinje cells. Yan and Johnson (1988) and Cohen-Cory et ah (1989) described and reviewed the development of NGF-R in rat cerebellum. Low affinity NGF-R immunoreactivity has been demonstrated with species-specific monoclonal antibodies in Purkinje cells of adult rats (Pioro and Cuello, 1988, 1990 Pioro et ah, 1991 Fusco et ah, 1991 Dusart et ah, 1994), monkey and human brain (Mufson et ah, 1991). Immunoreactivity was present in the somata, dendrites and the proximal axon of the Purkinje cells. Additional immunoreactivity in granule cells was reported by Vega et ah... 

Fig. 38. A. Nerve growth factor-R (NGF-R) transcripts are localized within Purkinje cells in the paraflocculus of rat cerebellum. B. NGF-R immunocytochemistry shows the perikarya of the Purkinje cells as well as the dense staining of the molecular layer, where the dendritic trees of the Purkinje cells arborize. Arrows in C and D point to parasagittal zones of intense labelling interdigitated with weaker labelling. Bar = 90 fim. Koh et al. (1989).

The epitopes recognized by Hawkes family of monoclonal antibodies known as the anti-Zebrins are localized on Purkinje cells (see Section 3.1.8.). Zonal patterns that are identical or very similar to Zebrin I and II have been described for the distribution of 5 -nucleotidase (see above), the p75 low affinity nerve growth factor receptor protein in the rat (Section 3.1.10., Fig. 38), protein kinase C delta (Fig. 133) (see Section 3.1.5.) and the B30 antibody of Stainier and Gilbert (1989) (see Section 3.1.8.). Immunoreactiv-ity in mouse Purkinje cells for an antibody against HNK is partially congruent with the Zebrin negative Purkinje cells, but Zebrin+/HNK-l- Purkinje cells also exist (Hawkes, 1992 Eisenman and Hawkes, 1993). The similarity between the Zebrin pattern and the transient zonal patterns in the development of the Purkinje cell specific marker L7 is discussed in Section 6.2. 

The Zebrin pattern is positively or negatively correlated with the distribution of other Purkinje cell markers, discussed by Hawkes (1992) and Leclerc et al. (1992), and in Sections 3.1.8. and 6.1.3. of this chapter. Notably, the distributions of PKC<5 (Chen and Hillman, 1993a) (Section 6.1.3.) and of nerve growth factor receptor protein (Section... 

Cohen-Cory S, Dreyfus CF and Black IB (1989) Expression of high- and low-affinity nerve growth factor receptors by Purkinje cells in the developing rat cerebellum. Exp. Neurol, 105, 104-109. 

Dusart 1, Morel MP, Sotelo C (1994) Parasagittal compartmentation of adult rat Purkinje cells expressing the low-affinity nerve growth factor receptor changes of pattern expression after a traumatic lesion. 

Martinez-Murillo R, Caro L, Nieto-Sampedro M (1993) Lesion-induced expression of low-affinity nerve growth factor receptor-immunoreactive protein in Purkinje cells of the adult rat. Neuroscience, 52, 587-593. 

Pioro EP, Cuello AC (1988) Purkinje cells of adult rat cerebellum express nerve growth factor receptor immunoreactivity light microscopic observations. Brain Res., 455, 182-186. 

Pioro EP, Ribeiro-da-Silva A, Cuello AC (1991) Similarities in tbe ultrastructural distribution of nerve growth factor receptor-like immunoreactivity in cerebellar Purkinje cells of the neonatal and colchicine-treated adult rat. J. Comp. Neurol, 305, 189-200. 

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