Pseudomonas mendocina

NMR spectra have been reported for the Rieske-type ferredoxins from Xanthobacter strain Py2 (88) and of toluene 4-monooxygenase from Pseudomonas mendocina (T4MOC) (88a) as well as for the water-soluble Rieske fragment from the bci complex of Paracoccus deni-trificans (ISFpd) (89). The spectra of these proteins are similar, which is consistent with the close structural relationship between the three proteins. In the reduced (paramagnetic) state, all three proteins show several hyperfine-shifted resonances between +83 and -16 ppm at 400 MHz or between 110 and +25 ppm at 300 MHz (Table X). 

Toluene/o-xylene monooxygenase in P. stutzeri strain 0X1 carried ont snccessive monooxygenation of o-xylene (Bertoni et al. 1998), and the tolnene-4-monooxygenase of Pseudomonas mendocina KRl and tolnene-3-monooxygenase of Ralstonia pickettii PKOl can hydroxylate benzene, tolnene, and o-xylene (Tao et al. 2004 Vardar and Wood 2004). 

Tao Y, A Fishman, WE Bentley, TK Wood (2004) Oxidation of benzene to phenol, catechol, and 1,2,3-trihydroxybenzene by toluene 4-monooxygenase of Pseudomonas mendocina KR 1 and toluene... 

Whited GM, DT Gibson (1991) Separation and partial characterization of the enzymes of the toluene-4-mono-oxygenase catabolic pathway in Pseudomonas mendocina KRl. J Bacterial 173 3017-3020. 

McClay K, SH Streger, RJ Steffan (1995) Induction of toluene oxidation in Pseudomonas mendocina KRl and Pseudomonas sp. strain ENVPC5 by chlorinated solvents and alkanes. Appl Environ Microbiol 61 3479-3481. 

Whited GM, DT Gibson (1991b) Toluene-4-monooxygenase, a three-component enzyme system that catalyzes the oxidation of toluene to p-cresol in Pseudomonas mendocina KRl. J Bacteriol 173 3010-3016. 

The transformation of A-nitrosodimethylamine by Pseudomonas mendocina KRl that has tolu-ene-4-monoxygenase activity was initiated by monooxygenation to the A-nitro compound, which produced A-nitromethylamine and formaldehyde, presumably by hydroxylation of the methyl group (Fournier et al. 2006). 

Fournier D, J Hawari, SH Streger, K McClay, PB Hatzinger (2006) Biotransformation of V-nitrosodunethyl-amine by Pseudomonas mendocina KRl. Appl Environ Microbiol 72 6693-6698. 

Nolan, L.C. and O Connor, K.E., Use of Pseudomonas mendocina, or recombinant Escherichia coli cells expressing toluene -monooxygenase, and a cell-free tyrosinase for the synthesis of 4-fluorocatechol from fluorobenzene. Biotechnol. Lett., 2007, 29, 1045. 

From Pseudomonas mendocina five siderophores were isolated by chromatography. They are reported to have identical molecular masses of 1,152 Da (the also reported 3a) value of 929 Da is an error L. E. Hersman, private communication) and an identical amino acid composition, which has not been revealed 141a). Color reactions show the presence of a hydroxamate, but not of a catecholate grouping. A gene analysis suggests a partial sequence acyl-Asp-Dab-Ser-formylOHOm-Ser-formylOHOm where asparagine could be OHAsp and the C-terminal ornithine cOHOm 9b). In which way the five isomeric siderophores with identical molecular masses differ from each other is not clear. 

Structural work may take its time. Examples are Pseudomonas mendocina (Sect. 2.9) where the first structural data were reported in 2000 and the next pertinent publication appeared in 2008, or Legionella pneumophila (Sect. 4.5) whose legiobactin was first characterized in 2000, further details followed in 2007 and 2009, with loose ends in both cases. Only partially characterized siderophores are mentioned wherever data were available in order to stimulate further work. This would be worthwhile siderophore research is a fascinating branch of natural products chemistry promising sometimes surprising results e.g. 311, 388)). 

Hersman LE, Huang A, Maurice PA, Forsythe JH (2000) Siderophore Production and Iron Reduction by Pseudomonas mendocina in Response to Iron Deprivation. Geomicrobiology J 17 261... 

Further work on p-menthane hydroxylation using Pseudomonas mendocina-SF (Vol. 6, p. 12) has resulted in isolating the metabolites (160 R=CH20H) and (160 ... 

Pseudomonas mendocina. In Annual Meeting of the American Society for Microbiology, abstr. K54, p. 156. St Louis, MI. 

Steffan, R. J. Tugusheva, M. (1993). Construction and selection of constitutive toluene monoxygenase (TMO) mutants of Pseudomonas mendocina KR1. In Abstracts, 4th International Symposium on Pseudomonas, abstr. p. 198. Vancouver, BC. 

Figure 16-18 Mossbauer X-ray absorption spectra of iron-sulfur clusters. (See Chapter 23 for a brief description of the method.) Quadrupole doublets are indicated by brackets and isomer shifts are marked by triangles. (A) [Fe2S2]1+ cluster of the Rieske protein from Pseudomonas mendocina, at temperature T = 200 K. (B) [Fe3S4]1+ state of D. gigas ferre-doxin II, T = 90 K. (C) [Fe3S4]° state of D. gigas ferredoxin II, T = 15 K. (D) [Fe4S4]2+ cluster of E. coli FNR protein, T = 4.2 K. (E) [Fe4S4]1+ cluster of E. coli sulfite reductase, T = 110 K. From Beinert et al.260...

Hersman L. E., Huang A., Maurice P. A., and Forsythe J. H. (2000) Siderophore production and iron reduction by pseudomonas mendocina in response to iron deprivation. Geomicrobiol. J. 17, 261-273. 

Maurice PA, Lee YJ, Hersman LE (2000) Dissolution of Al-substituted goethites by an aerobic Pseudomonas mendocina var. bacteria. Geochim Cosmochim Acta 64 1363-1374 McCoy JM, LaFemina JP (1997) Kinetic Monte Carlo investigation of pit formation at the CaCOs (1014) surface-water interface. Surface Sci 373 288-299... 

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