Pseudomonas fluorescent strain

Two sets of equations can be written, allowing the use of two indqtendent sets of XPS data to evaluate the proportion of carbon associated with protein (Cpr/C), polysaccharide (Cps/C), and hydrocarbonlike components (Chc/C). One scheme is based on observed elemental concentration ratios (obs), where the model protein chosen is the major outer protein of a Pseudomonas fluorescent strain (Table 5). 

Meyer JM, Stintzi A, Coulanges V, Shivaji S, Voss JA, Taraz K, Budzikiewicz H (1998) Siderotyping of Fluorescent Pseudomonads Characterization of Pyoverdines of Pseudomonas fluorescens and Pseudomonas putida Strains from Antarctica. Microbiology 144 3119... 

A green fluorescent protein-based Pseudomonas fluorescens strain biosensor was constructed and characterized for its potential to measure benzene, toluene, ethylbenzene, and related compounds in aqueous solutions. The biosensor is based on a plasmid carrying the toluene-benzene transcriptional activator (Stiner and Halverson, 2002). Another microbial whole-cell biosensor, using Escherichia coli with the promoter luciferase luxAB gene, was developed for the determination of water-dissolved linear alkanes by luminescence (Sticher et al., 1997). The biosensor has been used to detect the bioavailable concentration of alkanes in heating oil-contaminated ground-water samples. 

Fluorescence Studies of 4 octylstyrene and Poly[trialkyl-3-(and 4-) vinylbenzylammonium chloride] with Pseudomonas aeruginosa Strain PAOl... 

Incubation of Pseudomonas putida with anthracene-labeled carbon-base ferrichrome analog Fe(lll) complex 173 resulted in cellular iron uptake and the appearance of anthracene fluorescence in the culture medium identical to the Fe-ferrichrome uptake. Incubation with the alanyl analog 174 failed to show any significant iron uptake or fiuorescence. This is consistent with the tests described above on the unlabeled analogs. Remarkably, other strains such as Pseudomonas fluorescens S680 or WCS3742 also did not show any iron uptake or culture fluorescence. 

Many AB strains produce secondary metabolites that are inhibitory to plants, including phytotoxins and antibiotics, which can be considered allelopathic. Phytotoxins from fluorescent Pseudomonas spp., a diverse group of plant pathogenic bacteria abundant in the soil and rhizosphere, have been well studied (Mitchell, 1991). There are fewer reports on phytotoxins from AB and many have not been extensively studied. 

Antibiotic-producing fluorescent Pseudomonas strains have been readily isolated from soils that naturally suppress diseases such as take-all (a root and crown disease) of wheat, black root rot of tobacco, and fusarium wilt of tomato [145]. 

The production of HCN by Pseudomonas species has also been implicated in biological control. Hydrogen cyanide is a general biocide that chelates divalent cations and interferes with respiration by interaction with cytochrome c oxidase. Fluorescent pseudomonads are insensitive to cyanide especially as growth ceases and many strains with biocontrol activity can produce it in the presence of a suitable precursor such as glycine and Fe3+. A number of recent reviews have covered the topic of biological control by Pseudomonas in significant details [13-17]. 

Cho, I.-C., Tiedje, l.M. (2000). Biogeography and degree of endemicity of fluorescent Pseudomonas strains. Applied Environmental Microbiology 66, 5448-5456. 

Studies of pyocyanin (3) production in Pseudomonas strains have shown that the biosynthetic transformations from phenazine-1-carboxylic acid (Ih) to pyocyanin (3) are highly consistent in fluorescent Pseudomonas sp. First, the 5-methylphenazine-1-carboxylic acid betaine (59) is formed by methylation of the 5-position on phenazine-1-carboxylic acid, catalyzed by SAM-dependent (5-adenosyl-L-methion-ine) methyltranferase, and subsequently an NADPH-dependent flavoprotein monooxygenase catalyzes the hydroxylative decarboxylation (Scheme 3). ° 5-Meth-ylphenazine-1-carboxylic acid betaine (59) is also... 

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