Pseudomonas aeruginosa drugs against

Meropenem (Merrem IV) inhibits syndiesis of die bacterial cell wall and causes die deadi of susceptible cells. This drug is used for intra-abdominal infections caused by Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and odier susceptible organisms Meropenem also is effective against bacterial meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Hemophilus influenzae. 

It is obtained from Micromonospora pupurea. It has broader spectrum of action and is effective against Pseudomonas aeruginosa, E. coli, Klebsiella, Enterobacter and Proteus. Streptococci and enterococci are relatively resistant to it owing to failure of the drug to penetrate into the cell. Following parenteral administration, it defuses mainly into extracellular fluids. 

Palanichamy S, Ramakrishnan PN, Balasubramanian S, et al. Preservation of sodium chloride eye lotion BPC against contamination with Pseudomonas aeruginosa. Indian Drugs 1982 19 153-155. 

Examples of preservatives are phenylmercuric nitrate or acetate (0.002% w/v), chlorhexidine acetate (0.01% w/v), thiomersal (0.01% w/v) and benzalkonium chloride (0.01% w/v). Chlorocresol is too toxic to the corneal epithelium, but 8-hydroxy-quinoline and thiomersal may be used in specific instances. The principal consideration in relation to antimicrobial properties is the activity of the bactericide against Pseudomonas aeruginosa, a major source of serious nosocomial eye infections. Although benzalkonium chloride is probably the most active of the recommended preservatives, it cannot always be used because of its incompatibility with many compounds commonly used to treat eye diseases, nor should it be used to preserve eye-drops containing anaesthetics. As benzalkonium chloride reacts with natural rubbers, silicone or butyl rubber teats should be substituted and products should not be stored for more than 3 months after manufacture because silicone rubber is permeable to water vapour. As with all rubber components, the rubber teat should be pre-equilibrated with the preservative before use. Thermostable eye-drops and lotions are sterilized at 121 °C for 15 minutes. For thermolabile drugs, filtration sterilization followed by aseptic filling into sterile containers is necessary. Eye-drops in plastic bottles are prepared aseptically. 

This drug is particularly useful since it is effective against Pseudomonas aeruginosa. The new five-membered thiazolidine ring was incorporated, since the literature shows that it is advantageous in other cephalosporin systems. 

In vitro quantitative antibacterial activity was evaluated using Alamar Blue Assay [56]. It is a colorimetric oxidation-reduction assay which involves the addition of Alamar blue dye as an indicator. It evaluates the metabolic activity of the microorganisms. The activity of the evaluated drug is expressed as minimum inhibitory concentration ( 0,g/ml). Sampangine, SAMM2 and SAM MMl were tested for their activity against Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilus ATCC 6633, and E. coli ATCC 10536. Streptomycin was used as a positive control. 

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