Pseudomonas aeruginosa, contamination

Baird R.M. Shooter R.A. (1976) Pseudomonas aeruginosa infections associated with the use of contaminated medicaments. 2, 349-350. 

Noble W.C. Savin J. A. (1966) Steroid cream contaminated with Pseudomonas aeruginosa. Lancet,... 

Deschenes L, P Lafrance, J-P Villeneuve, R Samson (1996) Adding sodium dodecyl sulfate and Pseudomonas aeruginosa UG2 biosurfactants inhibits polycyclic hydrocarbon biodegradation in a weathered creosote-contaminated soil. Appl Microbiol Biotechnol 46 638-646. 

Increased removal of phenanthrene from soil columns spiked with the rhamnolipid mixture synthesized by Pseudomonas aeruginosa UG2 has been demonstrated, and shown to depend both on the increased desorption of the substrate and on partitioning into micelles (Noordman et al. 1998). However, the addition of the biosurfactant from the same strain of Pseudomonas aeruginosa UG2 or of sodium dodecyl sulfate had no effect on the rate of biodegradation of anthracene and phenanthrene from a chronically contaminated soil. 

The addition of a rhamnolipid biosurfactant produced by Pseudomonas aeruginosa stain ATIO apparently reduced the extent of degradation by endogenous bacteria of benz[fl]anthracene and chrysene in a creosote-contaminated soil (Vinas et al. 2005). 

The types of microorganisms found in various products are Pseudomonas species, including Pseudomonas aeruginosa, Salmonella, species, Staphylococcus aureus, and Escherichia coli. The USP and other pharmacopoeias recommend certain classes of products to be tested for specified microbial contaminants, e.g., natural plant, animal, and some mineral products for the absence of Salmonella species, suspensions for the absence of E. coli, and topically administered products for the absence of P. aeruginosa and S. aureus. Emulsions are especially susceptible to contamination by fungi and yeasts. Consumer use may also result in the introduction of microorganisms. For aqueous-based products, it is therefore mandatory to include a preservative in the formulation in order to provide further assurance that the product retains its pharmaceutically acceptable characteristics until it is used by the patient. 

In addition to the specific contaminant being objectionable, such contamination would be indicative of a deficient process as well as an inadequate preservative system. For example, the presence of a Pseudomonas putida contaminant could also indicate that P. aeruginosa, a similar source organism, is also present. 

Fluorescein in solution is highly susceptible to bacterial contamination, especially by Pseudomonas aeruginosa, which grows easily in the presence of fluorescein. Major methods of reducing the possibility of bacterial growth include sterile formulation and air-tight seal of solutions (e.g., injection fluorescein), use of effective... 

Contamination of fluorescein eye drops is a very serious risk (17). Pseudomonas aeruginosa is an especially dangerous pathogenic microorganism hable to invade fluorescein eye drops. Fluorescein is most safely dispensed in sterile single-dose units or as sterile fluorescein-impregnated paper strips. 

Palanichamy S, Ramakrishnan PN, Balasubramanian S, et al. Preservation of sodium chloride eye lotion BPC against contamination with Pseudomonas aeruginosa. Indian Drugs 1982 19 153-155. 

Endometritis in mares may be associated with at least four clinical s)mdromes. It can be caused by sexually transmitted infections, such as Taylorella equigenitalis (contagious equine metritis). Pseudomonas aeruginosa and Klebsiella pneumoniae. Pseudomonas and Klebsiella spp. may also be present in the fecal and genital flora. Chronic uterine infection with Streptococcus equi subsp. zooepidemicus and Escherichia coli are often associated with contamination of the uterus by fecal and genital flora. 

Limits for microbial contamination as set by the DCA. Pathogenic bacteria such as staph, aureus, pseudomonas aeruginosa, E.coli and salmonella should not be present. 

Pseudomonas aeruginosa Gram-negative, micro-aerobic rod General environmental contaminant Quintessential opportunist pathogen High resistance to antibiotics and biocides Biofilm-former... 

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