PSA-NCAM

PSA-NCAM, polysialic acid-neuronal cell adhesion molecule... 

PSA-NCAM Mouse IgM 1 500 Neuronal progenitors (Seki and Arai 1993) Dr. Tatsunori Seki... 

Fig. 12A-E Double-staining for BrdU and neuronal progenitor markers in SGZ. A An example of a BrdU/TUC4 double-stained cell (arrows) (day 9) adjacent to a BrdU+/TUC4 cell. B A BrdU+/Hu+ cell (day 23) with serial scanning at various depths to confirm the colocalization of the two channels. C A cell double-stained for Doublecortin (DCX) and BrdU (day 15) shown with channel separation. D A cell double-stained for /fill-tubulin and BrdU (day 9) extending processes parallel to DGL (arrowheads) is shown with channel separation. E A cell double-stained for PSA-NCAM and BrdU (day 44), extending processes parallel to DGL, is shown with channel separation. Scale bar= 10 pm...

Table 3 Percentages of colabeling of BrdU with neuronal progenitor markers in DG. BrdU+ cells were sampled for colabeling with any of /illl-lubulin, TUC4, Doublecortin, Hu, or PSA-NCAM as described in the text...

PSA-NCAM, an alternative progenitor cell marker, also colabeled with BrdU in SVZa (Fig. 49A arrows). Double-staining for PSA-NCAM and /3111-tubulin in SVZa... 

Fig. 49A-C (on page 70) Phenotype of PSA-NCAM+ cells in SVZa. A Double-staining for BrdU andPSA-NCAM in postischemic day-4 anterior SVZa. A large BrdU+ cluster is negative for PSA-NCAM (arrowheads). Nevertheless, some of the cells of an adjacent PSA-NCAM+ aggregate express BrdU (arrows the framed area is magnified in the lower panel). B Double-staining for PSA-NCAM and/3III-tubulin in postischemic day-23 anterior SVZa. Note almost complete colabeling. C Double-staining for PSA-NCAM and Nestin in postischemic day-9 caudate SVZa. Double-labeled cells are depicted by arrows. Asterisk, anterior horn of lateral ventricle. Scale bars = 100 pm (A, B) 50 pm (C)...

Immunostaining for BrdU and markers for immature migrating neurons (PSA-NCAM, /3111-tubulin or Doublecortin) in frontal white matter did not reveal chains... 

Fig. 51 Staining for PSA-NCAM, / III-tubulin, or Doublecortin in the subcortical white matter of postnatal (P14) or adult postischemic day-23 monkey brains. Note the lack of positive cells in the adult monkey white matter contrasting with the numerous positive clusters (left column arrows) in the postnatal brain. In adult brain, positive clusters were invariably in the vicinity of SVZa (right column arrows). Clusters depicted by arrows are magnified in the insets. The position of the visual field corresponds to the frame on the schematic map. F, frontal cortex WM, white matter S, striatum. Asterisk, anterior horn of lateral ventricle. Scale bar = 400 pm...

Adhesion molecules. The downregulation of the polysialylated neuronal cell adhesion molecule (PSA-NCAM) from the axonal surface (Charles et al 2000) is a necessary prerequisite to render the axon permissive to myelination (Charles et al., 2002 Coman et al., 2005). LI, another adhesion molecule expressed at the axonal surface, promotes myelination (Coman et al., 2005). 

In demyelination such as MS, PSA-NCAM is expressed on denuded axons and might act as an inhibitor of remyelination, whereas the myelinated part outside the plaque is PSA-NCAM negative (Charles et al., 2000 Coman et al., 2005). On the other hand, in MS a two- to threefold increase in OPC density and proliferation was found in the subventricular zone (SVZ), which correlated with enhanced numbers of PSA-NCAM(-i-) cells (Nait-Oumesmar et al., 2007). EAE in rodents is another important example of the activation of the SVZ and the involvement of progenitor cells expressing the polysialylated form of neural cell adhesion molecule (PSA-NCAM) in the repair process (Picard-Riera et al., 2002). 

Nomura T, Yabe T, Rosenthal ES, Krzan M, Schwartz JP (2000) PSA-NCAM distinguishes reactive astrocytes in 6-OHDA-lesioned substantia nigra from those in the striatal terminal fields. J Neurosci Res 61 588—596. 

Seki T (2002) Expression patterns of immatui e neui onal mai kers PSA-NCAM, CRMP-4 and NeuroD in die hippocampus of young adult and aged rodents. J Neui osci Res 70 327—334. 

Precursor A2B5- PSA-NCAM, Nestin, PDGFR-a, DM-20 Anti-PDGFR-a antibody PSA-NCAM+ /NestinV... 

Dey, P.M., M. Gochfield, and K.R. Reuhl. 1999. Developmental methylmercury administration alters cerebellar PSA—NCAM expression and Golgi sialytransferase activity. Brain Res. 845(2) 139-151. 

Durbec P, Cremer H (2001) Revisiting the function of PSA-NCAM in the nervous system. Mol Neurobiol 24 53-64... 

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