Proteomics protein identification and metastasis

Field Characterization of specific cell type proteomes. 

Methods Label-free mass spectrometry, shotgun proteomics. 

Reference A. M. Boutte, W. H. McDonald, Y. Shyr, and P. C. Lin, Characterization of the MDSC proteome associated with metastatic murine mammary tumor using label-free mass spectrometry and shotgun proteomics, PLoS One, 6 e22446 (2011). 

you have to admit that we are both pretty unpleasant characters. But at least 1 don t metastasize. 

Background Untreatable metastasis, rather than the primary tumor, is the cause of mortality in breast cancer. Myeloid-derived suppressor cells (MDSCs) are hema-topoetic cells that home specifically to the tumors and have a major role in tumor invasion and metastasis and the development of resistance to chemotherapy. MDSCs proliferate in response to tumors and accumulate in the spleen, from which they can be isolated using their Grl and CDllb surface markers. The objective was to use label-free mass spectrometry and shotgun proteomics to characterize MDSCs that associate with two mouse cell lines derived from the same tumor, one from the primary tumor (67NR) and the other from cells that have already metastasized to various organs (4T1). Spectral counting, for quantification, and protein network analysis were used to search for MDSC biomarkers characteristic to metastasis. 

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