Proteomics functional characterization

Staub I, Sieber SA (2009) P-Lactam probes as selective chemical-proteomic tools for the identification and functional characterization of resistance associated enzymes in MRSA. J Am Chem Soc 131 6271-6276... 

Proteomics The study of the proteins encoded by the genome, with focus on the high-throughput systematic separation, identification, and structural and functional characterization of proteins. 

Of paramount importance in future work will be the functional characterization of devices when in contact with complex proteomic samples, evaluating, among other things, biofouling, long-term operation, and the capacity to deliver the analytes of interest to the MS for analysis. In particular, the latter requires an efficient release of sample from MALDI targets or ionization... 

Modebadze, L, Rukhadze, M., Bakuradze, E., Dzidziguri, D. Pancreatic Cell Proteome - Qualitative Characterization and Function. Georgian Medical News, 7-8(220-221), 71-77,2013. 

Metalloproteomics is a new subject focusing on the distributions and compositions of all metalloproteins in a proteome (metalloproteome), their structural and functional characterization and their structural metal binding moieties. The specificity of metalloproteomics studies demands the need for a description of the metal-binding sites, metal stoichiometry, and metal-depen-dent structure or conformation changes as well as the identifieation and quantification of the metalloproteins. The metalloproteome can be considered as not only a subset of the metallome, but also a very important subset of the proteome. 

The study focusing on the distributions and 31,34 compositions of all metalloproteins in a proteome, their structural and functional characterization and their structural metal binding moieties... 

Post-translational modification of proteins plays a critical role in cellular function. For, example protein phosphorylation events control the majority of the signal transduction pathways in eukaryotic cells. Therefore, an important goal of proteomics is the identification of post-translational modifications. Proteins can undergo a wide range of post-translational modifications such as phosphorylation, glycosylation, sulphonation, palmitoylation and ADP-ribosylation. These modifications can play an essential role in the function of the protein and mass spectrometry has been used to characterize such modifications. 

Thus there is sufficient ground to cover at the functional level even after successful genetic characterizations that justifies the development of technologies for functional genomics, especially for high-throughput monitoring of proteomes and metabolomes. 

Pennington, S.R., Wilkins, M.R., Hochstrasser, D.F. and Dunn, M.J. (1999) Proteome analysis from protein characterization to biological function. Trends in Cell Biology, 1, 168-173. 

To facilitate our understanding of gene function, large-scale analyses of proteins are also necessary. Proteomics is currently divided into three main areas (1) mass spectrometry for characterization and identification of proteins (2) differential display proteomics for comparison of protein levels and (3) studies of protein-protein interaction by using the yeast two-hybrid system or phage display technology. 

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