Proteins biochemical isolation

The ion channel receptors are relatively simple in functional terms because the primary response to receptor activation is generated by the ion channel which is an integral part of the protein. Therefore, no accessory proteins are needed to observe the response to nicotinic AChR activation and the full functioning of the receptor can be observed by isolating and purifying the protein biochemically and reconstituting the protein in an artificial lipid membrane. In contrast, the G-protein-coupled receptors require both G-proteins and those elements such as phospholipase-C illustrated in Fig. 3.1, in order to observe the response to receptor activation (in this case a rise in intracellular calcium concentration resulting from the action of IP3 on intracellular calcium stores). 

J.V. Bannister, W.H. Bannister, and E.J. Wood, Bovine erythrocyte cupro-zinc protein. 1. Isolation and general characterization. Fur. J. Biochem. 18, 178-186 (1971). 

Bellavite, P., Bazzone, F., Cassatella, M. A., Hunter, K. J., Bannister, J. V. (1990). Isolation and characterization of a cDNA clone for a novel serine-rich neutrophil protein. Biochem. Biophys. Res. Commun. 170, 915-22. 

Lee, T. G. and Maruyama, S. (1998). Isolation of HTV-1 protease-inhibiting peptides from thermolysin hydrolysate of oyster proteins. Biochem. Biophys. Res. Commun. 253, 604-608. 

J. Sjoquist, B. Meloun and H. Hjelm, Protein A isolated from Staphylococcus aureus after digestion with lysostaphin, Eur. J. Biochem., 29 (1972) 572-578. 

Ihm, J., Harmony, J. A. K., Ellsworth, J., and Jackson, R. L., Simultaneous transfer of cholesteryl ester and phospholipid by protein(s) isolated from human lipoprotein-free plasma. Biochem. Biophys. Res. Common. 93, 1114-1120 (1980). 

Wober, W., Alaupovic, P. Studies on the protein moiety of endotoxin from Gram-negative bacteria. Characterization of the protein moiety isolated by acetic acid hydrolysis of endotoxin from Serratia marcescens 08. Eur J Biochem 19 (1971) 357-367. 

Huang, C. and Bems, D.S. 1983. Partial characterization of six chlorophyll a-protein complexes isolated from a blue-green alga by a non-detergent method. Arch. Biochem. Biophys., 220.145-154. 

JP Thornber, CA Smith and JL Bailey (1966) Partial characterization of two chlorophyll-protein complexes isolated from spinach-beet chloroplasts. Biochem J 100 14-15... 

The versatility and the potential of aqueous two-phase systems in future biotechnology has been amply demonstrated. The applications described here deal with extractive bioconversions, isolation and purification of proteins. Biochemical analyses in terms of binding assays have also been successfully applied in the two-phase systems... 

Fosang AJ, Hardingham TE. Isolation of the V-terminal globular protein domains from cartilage proeoglycans. Identification of G2 domain and its lack of interaction with hyaluronate and link protein. Biochem J 1989 261 801-809. 

Biochemical isolation of protein targets of a clinically used drug requires the chemical synthesis of its affinity resins. Preparation of the affinity resins is facilitated by information about the structure-activity relationships of the small... 

Murthy, P. V. H., Raghupathy, E., and Chaikoff, I. L., 1%5, Studies on thyroid proteins. I. Isolation and properties of a glycopeptide from sheep thyroglobulin, Biochem. 4 611-618. 

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