Protein precipitation liquid chromatography mass

Xue, Y. J., Liu, J., Pursley, J., and Unger, S. (2006). A 96-well single-pot protein precipitation, liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of muraglitazar, a novel diabetes drug, in human plasma. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 831 213 -222. 

Quantification of TKIs in patients plasma samples is at present principally performed by liquid chromatography-mass spectrometry (LC-MS) after suitable plasma pretreatment, which implies most generally a protein precipitation with an organic... 

Macek et al. [120] developed a method to quantitate omeprazole in human plasma using liquid chromatography-tandem mass spectrometry. The method is based on the protein precipitation with acetonitrile and a reversed-phase liquid chromatography performed on an octadecylsilica column (55 x 2 mm, 3 /im). The mobile phase consisted of methanol-10 mM ammonium acetate (60 40). Omeprazole and the internal standard, flunitra-zepam, elute at 0.80 0.1 min with a total rim time 1.35 min. Quantification was through positive-ion made and selected reaction monitoring mode at m/z 346.1 —> 197.9 for omeprazole and m/z 314 —> 268 for flunitrazepam, respectively. The lower limit of quantification was 1.2 ng/ml using 0.25 ml of plasma and linearity was observed from 1.2 to 1200 ng/ml. The method was applied to the analysis of samples from a pharmacokinetic study. 

Nielsen MK, Johansen SS (2009) Determination of olanzapine in whole blood using simple protein precipitation and liquid chromatography-tandem mass spectrometry. J Anal Toxicol 33(4) 212—217... 

Poison C et al (2003) Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 785 263-275... 

Taxotere) in Mouse Plasma by Protein Precipitation and High-Performance Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry, Using Paclitaxel as Internal Standard. ... 

Hou W, Watters JW, McLeod HL (2004) Simple and rapid docetaxel assay in human plasma by protein precipitation and high-performance liquid chromatography-tandem mass spectrometry. Journal of Chromatography B 804 263-267 Schuhmacher J, Zimmer D, Tesche F, Pickard V (2003) Matrix effects during analysis of plasma samples by electrospray and atmospheric pressure chemical ionization mass spectrometry practical approaches to their elimination. Rapid Communications in Mass Spectrometry 17 1950-1957 Shah PW (2001) Guidance for Industry Bioanalytical Method Validation U.S. Department of Health and Human Services, Food and Drug Administration... 

High-performance liquid chromatography-tandem mass spectrometry method for the determination of gemifloxacin in human plasma was based on the protein precipitation of plasma samples with acetonitrile containing [ C Ha] gemifloxacin as an internal standard. The supernatant was injected onto a PLRP-S column without any further clean-up. The mass spectrometer was operated in positive ion mode, and the ions were detected in multiple reaction-monitoring (MRM) mode. The assay requires 50 gl of plasma and is precise and accurate within the range 10-5000 ng/ml [15]. 

Polson, C. Sarkar, P Incledon, B. Raguvaran, V. Grant, R. Optimization of Protein Precipitation Based upon Effectiveness of Protein Removal and Ionization Effect in Liquid Chromatography-Tandem Mass Spectrometry, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 785, 263-275 (2003). 

SS. Murphy, A.T Berna, M.J. Holsapple, J.L. Ackermann, B.L. Effects of Flow Rate on High-Throughput Quantitative Analysis of Protein-Precipitated Plasma Using Liquid Chromatography/Tandem Mass Spectrometry, Rapid Commun. Mass Spectrom. 16, 537-543 (2002). 

Marney LC, Laha TJ, Baird GS, Rainey PM, Hoofnagle AN. Isopropanol protein precipitation for the analysis of plasma free metanephrines by liquid chromatography— tandem mass spectrometry. Clin Chem 2008 54 1729—32. 

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