Protein engineering process using sequence activity

Figure L Protein engineering process using sequence activity relationships.

Since it appears possible to use active biomolecules outside a natural environment, and even more so since genetic engineering enables the production by recombinant microorganisms or animal cell cultures of more and more various proteins, a great deal of investigation has been carried out in order to separate and purify these compounds from complex medium broths. Due to the continually renewed diversity and complexity of proteins and media, a simple scheme of extraction and purification process does not exist, each example being a specific case for which, usually, a sequence of several techniques must be developed, depending on the properties of the protein, the nature of the impurities and the final purity demanded. 

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