Protein chemistry techniques

West, K.A. and Crabb, J.W 1990 Performance evaluation automatic hydrolysis and PTC amino acid analysis. In Vallafraca, J.J., ed.. Current Research in Protein Chemistry Techniques, Structure, and Function. San Diego, Academic Press 37-48. 

Because lantibiotics are gene-encoded antimicrobials, genetic, molecular biology, and protein chemistry techniques have been used traditionally to explore their structure and biosynthesis. These approaches include gene deletions and disruptions, and... 

These methods are based on the use of light waves to probe the macromolecular nature of ion channels and are useful for obtaining structural information about ion channel proteins. Without enough valid structural information, all data gleaned from traditional protein chemistry techniques are simply... 

The identity of any newly expressed recombinant protein should be venfied and its structural integrity demonstrated before pursuing structure-function studies see Note 8). Several protein-chemistry techniques have proven useful for characterizing rCRALBP. 

This chapter lists some representative examples of biochemicals and their origins, a brief indication of key techniques used in their purification, and literature references where further details may be found. Simpler low molecular weight compounds, particularly those that may have been prepared by chemical syntheses, e.g. acetic acid, glycine, will be found in Chapter 4. Only a small number of enzymes and proteins are included because of space limitations. The purification of some of the ones that have been included has been described only briefly. The reader is referred to comprehensive texts such as the Methods Enzymol (Academic Press) series which currently runs to more than 344 volumes and The Enzymes (3rd Edn, Academic Press) which runs to 22 volumes for methods of preparation and purification of proteins and enzymes. Leading referenees on proteins will be found in Advances in Protein Chemistry (59 volumes. Academic Press) and on enzymes will be found in Advances in Enzymology (72 volumes, then became Advances in Enzymology and Related Area of Molecular Biology, J Wiley Sons). The Annual Review of Biochemistry (Annual Review Inc. Patio Alto California) also is an excellent source of key references to the up-to-date information on known and new natural compounds, from small molecules, e.g. enzyme cofactors to proteins and nucleic acids. 

Leach, S. J., Ed., Physical Principles and Techniques of Protein Chemistry, Academic Press, New York, 1969. 

The history of protein chemistry can be summarised as improved purification techniques combined with new methods of protein characterisation allied to theoretical interpretation of the results obtained. Improved computational techniques have also helped considerably. 

Principles and techniques of protein chemistry. Leach, S. J. (ed.) New York Academic Press 1969... 

Jossang, P. (1992). Origins of chromatography. Nature, London 356, 100 Leggat Baily, J. (1962). Techniques in Protein Chemistry. Elsevier, Amsterdam. 

Legget-Bailev, J. Techniques in protein chemistry. Elsevier Publishing Co., 1962. 

Corresponding improvements in the semisynthesis of proteins have been developed (see Section 5.1.11). They, too, have involved intermolecular and intramolecular reactions to form normal peptide bonds between small synthetic peptides and large protein components derived from natural sources or produced by recombinant techniques. In this case, there is the potential to produce derivatives of much larger proteins than can currently be prepared by total chemical synthesis. The total synthesis and semisynthesis approaches are useful for somewhat different purposes, but can be considered complementary together, they greatly broaden the field of protein chemistry and biology. 

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