Protease inhibitors, composition

Aldamiz-Echevarria L, Pocheville I, Sanjurjo P, Elorz J, Prieto JA, Rodriguez-Soriano J. Abnormalities in plasma fatty acid composition in human immunodeficiency virus-infected children treated with protease inhibitors. Acta Paediatr 2005 94(6) 672-7. 

Peptide derivatives, (II), prepared by Saksena (3) were effective as NS3 serine protease inhibitors of hepatitis C when used in pharmaceutical compositions containing a-interferon or pegylated interferon. 

Labeque (Procter Gamble Co.) et al. [68,69] proposed the use of a low level of peptide trilluoromethyl ketones as reversible protease inhibitors in aqueous liquid detergent composition. This approach is attractive and particularly critical in the formulation of concentrated liquid detergent compositions. 

R. Schmidt and G. Winter, Wound dressing compositions, especially for dehvery of protease inhibitors, AU2005291398, June 28, 2012. 

DSC has been used to study the individual protein components of biological membranes of relatively simply protein composition and the interaction of several of these components with lipids and with other proteins. The red blood cell membrane, which has been most intensively studied, exhibits five discrete protein transitions, each of which has been assigned to a specific membrane protein. The response of each of these thermal transitions to variations in temperature and pH as well as to treatment with proteases, phospholipases, specific labelling reagents, and modifiers and inhibitors of selected membrane activities, has provided much useful information on the interactions and functions of these components in the intact erythrocyte membrane (46-49). Similar approaches have been applied to the bovine rod outer segment membrane (50) and to the spinach chloroplast thylakoid membrane (51). 

With the earlier discovery of the tetrameric composition of human yS-tryptase [83], various classes of bibasic inhibitors have first been synthesized rather in a trial- and error-manner. Later, a structure-based approach could be applied based on the exact geometry of the tetrameric protease [74, 75]. Among the various genera-... 

The inhibitor has been purified using a modification of the scheme of Millet and Gregoire [2] (Table 2) plus a final fractionation on a reverse phase HPLC column employing an n-butanol gradient (in 0.1% trifluoroacetic acid). The amino acid composition differs somewhat from inhibitors of E. coli or Streptomyces (Table 3) but they are probably all of the same general class in that they contain a low percentage of cysteine and are active on either subtilisin (E. coli and Streptomyces) or related serine proteases [8],... 

An archaebacterial acid protease (optimum pH 2.0) which may represent a new class of enzyme has been purified recently from Sulfolobus acidocaldarius [297, 298]. Although the specificity of the enzyme was similar to pepsin, with preference shown towards bonds between large hydrophobic residues, inhibitor studies indicated differences in active site composition. Furthermore, the nucleotide sequence of the enzyme gene was completely dissimilar to those for enzymes of the pepsin family and to pepstatin-insensitive acid protease. 

Changes in the ratio of activity of proteases and their inhibitors are observed in the fungal and bacterial skin lesions, traumatic lesions of the eye, bums, apoptosis and other pathological processes (Garcia-Carreno, 1996 Bohm et al., 1998 Thorbum et al., 2003 Wouters et al., 2008). Thus, some inhibitors are actively affected both the cellular environment and on the cell proteome, changing its composition (Vincent Zivy, 2007). 

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