Propagation micropropagation

Micropropagation the provision of plants difficult to propagate using normal methods or those of endangered species... 

The plant cell culture technique of micropropagation of flavour-producing plants will be able to help with their agricultural cultivation and will relieve the pressure on the wild populations. Micropropagation will be able to propagate those plants where conventional propagation is difficult or will be to multiply elite stock. This may be required if demand for natural flavours continues to increase. 

Plant micropropagation is another important application of plant cell culture, which is an efficient method of propagating disease-free, genetically uniform... 

In the meantime, the formation of the main alkaloids in C. ipecacuanha under a variety of conditions has been extensively investigated emetine (1) in callus cultures (49) and under the effects of L-tyrosine supplementation (5t)) emetine (1) and cephaeline (2) in Panamanian ipecac (57), in Nicaraguan ipecac (52), in regenerates obtained by clonal propagation (53,54), in tissue cultures (55) and under the effects of exogenous feeding of shikimic acid and L-phenylalanine (55), in cell suspension and excised root cultures (57), in adventitious root cultures (58), and in callus cultures (56,59) and the effects of age and electrokinetic potential (60) ipecoside (7) in the roots (61) and the effect oi Azotobacter, leaf mold, and farmyard manure on alkaloid content (62). In addition, micropropagation systems for C. ipecacuanha have been developed (63-65). 

Rauvolfia serpentina roots are the source used in industrial extraction of the alkaloids. The plant is propagated by root cuttings as it has low seed viability (697). The use of micropropagation is thus of interest, and several authors reported successful procedures for micropropagation (698-701). Mathur et al. (697) reported the micropropagation of colchicine-induced tetraploids. Multiple shoot formation was obtained, as reported by Roja et al. (688), on MS medium containing 1 ppm BAP and 0.1 ppm NAA. 

Therefore, alternative approaches for the propagation of improved planting materials must be considered and in vitro propagation or micropropagation via somatic embryogenesis seems to provide a convenient alternative for the future due to its potential for massive propagation. 

---