Probes cellular imaging

Probes for Cellular Imaging, Proteins, and Other High-Molecular-Weight Analytes... 

Umezawa Y (2005) Genetically encoded optical probes for imaging cellular signaling pathways. Biosens Bioelectron 20 2504-11... 

The tetraazatriphenylene chromophore attached to the cyclene ring acted as an efficient sensitiser for Eu3+ and Tb2+ emission but also intercalated between the base pairs of DNA. The complexes were tested as cellular imaging and reactive probes using the mouse fibroblast cell line. The complexes were quickly taken up by the fibroblast cells and localised in nucleus and around the cell membrane. The process was visualised by fluorescence microscopy. Photolysis at 340 nm and 350 nm damaged plasmid supercoiled DNA producing nicked (form II) and linear (form III) DNA. DNA damage is known to induce apoptotic cell death and these complexes may be therefore considered for the development as therapeutic probes, for example in the treatment of accessible tumours, such as skin melanoma. 

Frias, J. C., Bobba, G., Cann, M. J., Hutchison, C. J., Parker, D. Luminescent nonacoordinate cations lanthanide complexes as potential cellular imaging and reactive probes, Org. Biomol. Chem. (2003), 905... 

Vandevyver, C.D.B., Chauvin, A.S., Comby, S., and Biinzli, J.C.G (2007) Luminescent lanthanide bimetalUc triple-stranded helicates as potential cellular imaging probes. Chemical Communications, 1716-1718. 

Organic dye materials represent the largest and best characterized class of probes used in all manner of fluorescent analysis. As an overall class, these dyes are used in almost all areas of biotechnology, including biosensing, cellular imaging, clinical immunofluorescence, and in DNA/protein microarrays (42-45). Several major structural classes of organic fluorophore span the UV-to-near-IR spectrum see Fig. 4. UV dyes are typically pyrene-based, naphthalene-based, and coumarin-based structures, whereas the Vis/near-IR dyes include a variety of... 

Abstract. Electroanalytical methods are highly compatible with micro- and nano-machining technology and have the potential of invasive but non-destmctive cell analysis. In combination with optical probes and imaging techniques, electroanalytical methods show great potential for the development of multi-analyte detection systems to monitor in real-time cellular dynamics. 

Ruthenium(II) polypyridyl complexes and DNA From structural probes to cellular imaging and therapeutics 12CSR3179. 

Cellular imaging has become a topic of intensive research in recent years, and luminescent metal-based probes have been intensively utilized for such a process. The transition metal ions of ruthenium(II), rhenium(I), and iridium(III) have been particularly effective as luminescent intracellular imaging agents owing to their kinetic inertness (low rates of ligand exchange), attractive photophysical properties (large... 

Bohme, R. et al (2010) Biochemical imaging below the diffraction limit — probing cellular membrane related structures by Tip-Enhanced Raman Spectroscopy (TERS). J. Biophotonics, 3, 455-461. doi ... 

Thibon A, Pierre VC. Principles of Responsive Lanthanide-Based Luminescent Probes for Cellular Imaging. Anal Bioanal Chem 2009 394 107-120. 

Most disease-specific molecular and cellular processes involve low concentrations and have low capacities. Therefore, molecular imaging requires highly sensitive imaging modalities to detect and quantify the imaging probes as it is done with short-lived radioisotopes. Optical imaging and ultrasound are next in sensitivity. MRI would require too high contrast agent concentrations... 

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