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Precipitin reactions between

Quantitative precipitin tests were performed as described.73 The amounts of precipitates obtained at the various concentrations were measured by the protein phenol method.74 The inhibition values are recorded in Table I. These inhibition data show that the precipitin reaction between the tetrahet-eropolysaccharide and the anti-GlcA antibodies is strongly inhibited by d-glucuronic acid, but with the exception of galacturonic acid none of the other carbohydrates were inhibitory. The data in the table also show that the carbohydrates tested did not inhibit the precipitin reaction between the polysaccharide and anti-GlcA-Rha antibodies. The monosaccharides alone cannot completely fit the active site of this antibody to give a precipitin test. [Pg.238]

Ions of Mn2+ and Ca2+ were bound to the purified, lima-bean lectins.151,199 Removal of Mn2+ lowered the hemagglutination titer by 75%. (Ethylenedinitrilo)tetraacetate completely inhibited the precipitin reaction between lima-bean lectin component III and type A blood-group substance (compare Ref. 579). Several divalent-metal cations restored activity to the demetallized protein or (ethylenedinitrilo)tetraacetate-treated lectin the addition of Ca2+,... [Pg.247]

The immunochemical studies of Kabat and coworkers200 686 revealed some striking subtleties in Lotus tetragonolobus sugar-specificity. Precipitin reactions between the lectin and several blood-group H substances demonstrated strong affinity of the lectin for this determinant. H substances precipitated 83-88% of the purified Lotus lectin at... [Pg.285]

Sugar Inhibition2"0 of the Precipitin Reaction Between Human H Substance and Lotus tetragonolobus Lectins A, B, and C... [Pg.286]

Fig. 5. Quantitative precipitin reaction between crystalline hen egg albumin (Ea) and rabbit antibody (Ab) to crystalline hen egg albumin. From Heidelberger and Kendall. ... Fig. 5. Quantitative precipitin reaction between crystalline hen egg albumin (Ea) and rabbit antibody (Ab) to crystalline hen egg albumin. From Heidelberger and Kendall. ...
Figure 7. Inhibition by oligosaccharides of the precipitin reaction between ACl-B and anti-ACE serum (O) trisaccharides, (A) tetrasaccharides, pentasaccharides, (%) hexasaccharides, ( ) heptasaccharides, (] ) octasaccharides. Reaction system 200 jxL antiserum (two-fold diluted), 150 fig ACI-B, and 200 fiL inhibitor were mixed total volume of reaction mixture was 500 fiL. Maximum addition for the hexa-, hepta-, and octasaccharides was 4 fimol because they were in limited quantities. (25)... Figure 7. Inhibition by oligosaccharides of the precipitin reaction between ACl-B and anti-ACE serum (O) trisaccharides, (A) tetrasaccharides, pentasaccharides, (%) hexasaccharides, ( ) heptasaccharides, (] ) octasaccharides. Reaction system 200 jxL antiserum (two-fold diluted), 150 fig ACI-B, and 200 fiL inhibitor were mixed total volume of reaction mixture was 500 fiL. Maximum addition for the hexa-, hepta-, and octasaccharides was 4 fimol because they were in limited quantities. (25)...
Figure 3. Quantitative precipitin reactions between the mannan subfractions (Batch I) and antisera of three C. albicans strains and S. cerevisiae (A) anti-C. albicans NIH A-207 (A-strain) serum (B) anti-C. albicans NIH B-792 (B-strain) serum (C) anti-C. albicans J-1012 (J-strain) serum (D) anti-S. cerevisiae serum. Mannan subfractions are of (O) A-strain (0) B-strain (A) J-strain (A)... Figure 3. Quantitative precipitin reactions between the mannan subfractions (Batch I) and antisera of three C. albicans strains and S. cerevisiae (A) anti-C. albicans NIH A-207 (A-strain) serum (B) anti-C. albicans NIH B-792 (B-strain) serum (C) anti-C. albicans J-1012 (J-strain) serum (D) anti-S. cerevisiae serum. Mannan subfractions are of (O) A-strain (0) B-strain (A) J-strain (A)...
L. Jacobsson and R.Wilstrom, The Precipitin Reaction Between Naturally Occurring Human Dextran-reacting Antibodies and Cr Tagged Dextran, Scand. J. din. Lab. Invest. 15, 114-120 (1963). [Pg.377]

L.C. Wetter, M.Cohn and H.F.Deutsch, Immunological Studies of Egg-white Proteins. II. Resolution of the Quantitative Precipitin Reaction between Chicken Egg-white and Rabbit Anti-egg-white Serum in Terms of the Reactions of Purified Egg-white Proteins, J. Immun. 69, 109-115 (1952). [Pg.392]

Table 2. Inhibition by oligoinosinic acids of the precipitin reaction between anti-poly I poly C serum 201 and poly I poly C or poly I... Table 2. Inhibition by oligoinosinic acids of the precipitin reaction between anti-poly I poly C serum 201 and poly I poly C or poly I...
Table 5. Inhibition by oligoadenylic acids of the precipitin reaction between antipoly A poly U serum 907 and anti-poly A poly U or poly A... Table 5. Inhibition by oligoadenylic acids of the precipitin reaction between antipoly A poly U serum 907 and anti-poly A poly U or poly A...
See other pages where Precipitin reactions between is mentioned: [Pg.178]    [Pg.178]    [Pg.92]    [Pg.109]    [Pg.140]    [Pg.179]    [Pg.200]    [Pg.25]    [Pg.89]    [Pg.101]    [Pg.103]    [Pg.278]   
See also in sourсe #XX -- [ Pg.104 ]



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