Potassium hydrogen phosphate, solution

Phosphate buffer 0.1 mol L , pH — 7.0 solution is used as electrolytic solution. Crystals of potassium hydrogen phosphate K2HP04 and potassium dihydrogen phosphate KH2P04 are purchased from Sigma-Aldrich (France). All the reagents mentioned are of analytical grade. 

Considering water based electrolytes, Grimes et al. [34] achieved the best results (i.e., nanotubes up to 6 pm long) in a solution containing either fluoride salts (NaF or KF) or HF in the presence of buffer salts like sodium sulfate or sodium or potassium hydrogen phosphate in a pH interval ranging from 3 to 5 (Fig. 19). 

Photosynthetic membranes were washed with 2 iriM MES, pH 6.0 to remove phycocyanin and then washed with 1 M NaCl. This NaCl solution containing the cytochrome and residual phycocyanin was dialyzed against 1 mM Tris HCl, pH 7.5, and applied to a DEAE-cellulose column. The phycocyanin and cytochrome both bound, and were eluted in that order with a concentration gradient (potassium hydrogen phosphate, pH 6). 

Cjg, Abs = 284.06 x The data presented in this paper is the drug release in a phosphate buffer solution. The buffer solution was prepared by dissolving potassium hydrogen phosphate (0.8wt.%, Wako Chemicals) and sodium dihydrogen-phosphate (1.5wt.%,Wako Chemicals) in pure water. 

Freeman and Morrison (1949) have given a detailed procedure for the production of this antibiotic from T. roseum F227. The medium contained 50g of glucose, 2 g of ammonium tartrate, 0.5 g of magnesium sulfate, 1 g of potassium hydrogen phosphate, 0.5 g of potassium chloride, 0.01 g of ferrous sulfate, 10 ml of corn steep liquor, and sufficient water to make 1000 ml of solution. After the pH was adjusted to 5, the culture was allowed to stand for 28 days at 25 , filtered, the filtrate extracted with chloroform, and the chloroform evaporated. Final purification was effected by chromatography on alumina, and gave about 36 mg of trichothecin (I) per 1 of culture filtrate. 

To prepare the standard pH buffer solutions recommended by the National Bureau of Standards (U.S.), the indicated weights of the pure materials in Table 8.15 should be dissolved in water of specific conductivity not greater than 5 micromhos. The tartrate, phthalate, and phosphates can be dried for 2 h at 100°C before use. Potassium tetroxalate and calcium hydroxide need not be dried. Fresh-looking crystals of borax should be used. Before use, excess solid potassium hydrogen tartrate and calcium hydroxide must be removed. Buffer solutions pH 6 or above should be stored in plastic containers and should be protected from carbon doxide with soda-lime traps. The solutions should be replaced within 2 to 3 weeks, or sooner if formation of mold is noticed. A crystal of thymol may be added as a preservative. 

The following procedure is described in U.S. Patent 2,837,464 from a solution of 3 grams of yeast extract (Difco) in 3.0 (iters of tap water containing 13.2 grams of potassium dihydrogen phosphate and 26.4 grams disodium hydrogen phosphate (pH of the solution, 6.9)... 

Harrison, Tarr and Hibbert96 investigated the production of levan from sucrose by the action of Bacillus subtilis Cohn and B. mesentericus Trevisan. Nutrient solutions containing 10% carbohydrate, 0.1% peptone, 0.2% disodium hydrogen phosphate and 0.5% potassium chloride were incubated at 37° for six days. Levan formation occurred only with sucrose and raffinose, and not with melezitose, lactose, maltose, D-xylose, D-glucose or D-fructose. It was therefore suggested that only those carbohydrates with a terminal D-fructofuranose residue were satisfactory substrates for levan formation. 

Cabon tetrachloride, n-hexane, chloroform, ACN, acetone, THF, pyridine, acetic acid, and their various mixtures were applied as mobile phases for adsorption TLC. Methanol, 1-propanol, ACN, acetone, THF, pyridine and dioxane served as organic modifiers for RP-TLC. Distilled water, buffers at various pH (solutions of and dipotassium hydrogen phosphate or potassium dihydrogen phosphate) and solutions of lithium chloride formed the aqueous phase. Carotenoids were extracted from a commercial paprika sample by acetone (lg paprika shaken with 3 ml of acetone for 30 min), the solution was spotted onto the plates. Development was carried out in a sandwich chamber in the dark and at ambient temperature. After development (15 cm for normal and 7cm for HPTLC plates) the plates were evaluated by a TLC scanner. The best separations were realized on impregnated diatomaceous earth stationary phases using water-acetone and water-THF-acetone mixtures as mobile phases. Some densitograms are shown in Fig.2.1. Calculations indicated that the selectivity of acetone and THF as organic modifiers in RP-TLC is different [14],... 

Disodium hydrogen phosphate dihydrate (25.5 g), potassium dihydrogen phosphate (9.0 g), ammonium chloride (1.0 g) and sodium chloride (0.5 g) were dissolved in water and the volume adjusted to 900 mL. The solution was sterilized by autoclaving (121 °C, 20 min) and allowed to cool to room temperature. Magnesium sulfate (240.5 mg), kanamycin (50 mg), thiamine (10 mg) and glucose (5 g) were dissolved in water and adjusted to 100 mL volume. This mixture was sterilized by filtration through a 0.2 pm filter and added to the salt solution. 

X)8 695 m potassium dihydmgen phosphate. 0.030 43 m disodium hydrogen phosphate. Prepare like Buffer 5 dissolve 1.179 g KH2PO4 and 4.30 g Na2HPOA in water to give 1L erf solution at 25°C. 

Immobilized TEMPO has been used for the one-pot oxidation of alcohols to carboxylic acids as well.26 For this purpose TEMPO resin 1 was combined with two ion-exchange resins loaded with chlorite anions and hydrogen phosphate in the presence of catalytic amounts of potassium bromide and sodium hypochlorite in solution. The reaction required work-up for the removal of salts, but tolerated several protecting schemes and afforded pure products in good to excellent yields. The reaction is initiated by catalytic TEMPO oxidation of alcohols to aldehydes driven by dissolved hypochlorite followed by oxidation to the carboxylic acids effected by chlorite. 

Ammonia solution Disodium hydrogen phosphate Potassium hexacyanoferrate (II)... 

The System Lime and Phosphoric Acid —Solubility of Calcium Phosphates— Conditions of Formation of Basic and Acid Calcium Phosphates—Composition of Solutions Saturated with Calcium Hydrogen Phosphates—Equilibria between Solid Phases and Solutions at Various Temperatures—Changes during Neutralisation—The Acid Phosphates—Manufacture of Superphosphate—Retrogression—Treatment of Special Ores—Phosphoric Acid— Commercial Preparation and Extraction from Rook—The History and Technology of Superphosphate Manufacture—Mixed and Concentrated Phosphorio Fertilisers—Potassium Phosphates—Ammonium Phosphates. 

P-E-M Buffer Dissolve 8.8 g of potassium dihydrogen phosphate (KH2PO4) and 8.0 g of dipotassium hydrogen phosphate trihydrate (K2HP04-3H20) in about 900 mL of water. Add 10.0 mL of Magnesium Solution and 10.0 mL of EDTA Solution. Transfer the solution into a 1000-mL volumetric flask, dilute to volume with water, and mix. The pH should be 6.50 0.05. 

Solutions Hepes buffer. Hepes (20 mM), sodium chloride (135 mM), potassium chloride (5 mM), magnesium chloride (1 mM), disodium hydrogen phosphate (1 mM), glucose (5 mM), pH 7.40, osmolarity 300 mOsm/kg H20. 

The interaction of the calculated quantities of phosphoric acid and potassium carbonate in aqueous solution yields dipotassium hydrogen phosphate, KgHPO. It is only known in solution, and in this form has a slightly alkaline reaction to litmus,. but is neutral to phenolphthalein. Its heat of formation in aqueous solution is 429-2 Cal.4... 

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