Dipotassium hydrogen phosphate

Characteristic properties of endopectate lyases are the high pH optimum, and a requirement for Ca2+ ions in order to maintain catalytic activity. The pH optimum of various endopectate lyases ranges from 8.0 to 9.5 (Refs. 4, 178, 234, 236, 243). Besides activation by Ca2+ ions, the optimal concentration of which is 1 mM,234,236,244 strontium salts were also considered in the case of Bacillus sp. lyase.234 The enzyme from Pseudomonas sp. was also partly activated by magnesium chloride,178 and for the lyase of Clostridium felsineum, salts of other bivalent cations had an activating effect as well.245 (Ethylenedinitrilo)tetraacetic acid completely inactivated all of the lyases mentioned. The activity of endopectate lyase from Pseudomonas was also lessened in the presence of sodium chloride, potassium chloride, and dipotassium hydrogen phosphate (K2HP04). 

Materials Required Stilbosterol 20 mg ethyl alcohol (absolute) 250 ml dipotassium hydrogen phosphate solution (dissolve 1 g in 55 ml of DW) 25 ml ... 

Cabon tetrachloride, n-hexane, chloroform, ACN, acetone, THF, pyridine, acetic acid, and their various mixtures were applied as mobile phases for adsorption TLC. Methanol, 1-propanol, ACN, acetone, THF, pyridine and dioxane served as organic modifiers for RP-TLC. Distilled water, buffers at various pH (solutions of and dipotassium hydrogen phosphate or potassium dihydrogen phosphate) and solutions of lithium chloride formed the aqueous phase. Carotenoids were extracted from a commercial paprika sample by acetone (lg paprika shaken with 3 ml of acetone for 30 min), the solution was spotted onto the plates. Development was carried out in a sandwich chamber in the dark and at ambient temperature. After development (15 cm for normal and 7cm for HPTLC plates) the plates were evaluated by a TLC scanner. The best separations were realized on impregnated diatomaceous earth stationary phases using water-acetone and water-THF-acetone mixtures as mobile phases. Some densitograms are shown in Fig.2.1. Calculations indicated that the selectivity of acetone and THF as organic modifiers in RP-TLC is different [14],... 

Dipotassium hydrogen phosphate trihydrate (15.9 g) potassium dihydrogen phosphate (4.0 g) disodium hydrogen phosphate dodecahydrate (7 g) ammonium sulfate (1.2 g) ammonium chloride (0.2 g) magnesium sulfate heptahydrate (1 g) yeast extract (5 g )... 

Synonyms dipotassium hydrogen phosphate dipotassium phosphate potassium hydrogen phosphate. 

Per vial 8.32mg tranexamic acid 136.24mg dipotassium hydrogen phosphate 51.27 mg phosphoric acid 364 mg sucrose 5.2 mg polysorbate 80... 

Screening of ENRO, CIPRO, DAN, NOR, FLU, OXO, and NALA in pork muscle was based on HPTLC after the SPE procedure (195). Tissue samples were extracted with MeCN-NaOH, the supernatant dried, and the residues dissolved in dipotassium hydrogen phosphate buffer (pH 7.4) hexane was added. The aqueous phase was cleaned up using the SPE technique. After washing the cartridge, analytes were eluted with MeOH-ammonia solution (75 25). After the preparation step, extracts were spotted and eluted on silica gel plates. The plate is first inspected under UV illumination at 312 nm and then sprayed with terbium chloride. The method was validated at the levels of 15 yug/kg for ENRO, CPRO, DAN, and NOR and 5 yug/kg for FLU, 0X0, and NALA. 

Streptomyces narbonensis var. josatny ceticus Dipotassium hydrogen phosphate Magnesium sulfate Hydrochloric acid... 

P-E-M Buffer Dissolve 8.8 g of potassium dihydrogen phosphate (KH2PO4) and 8.0 g of dipotassium hydrogen phosphate trihydrate (K2HP04-3H20) in about 900 mL of water. Add 10.0 mL of Magnesium Solution and 10.0 mL of EDTA Solution. Transfer the solution into a 1000-mL volumetric flask, dilute to volume with water, and mix. The pH should be 6.50 0.05. 

The interaction of the calculated quantities of phosphoric acid and potassium carbonate in aqueous solution yields dipotassium hydrogen phosphate, KgHPO. It is only known in solution, and in this form has a slightly alkaline reaction to litmus,. but is neutral to phenolphthalein. Its heat of formation in aqueous solution is 429-2 Cal.4... 

Potassium pyrophosphate, K4P207.—The pyrophosphate is produced by heating dipotassium hydrogen phosphate, or by neutralizing anhydrous phosphoric acid with potassium hydroxide dissolved in absolute alcohol. Its specific heat is 0-1910 between 17° and 98° C.11 It is soluble in water, forming a slightly alkaline solution, and yields a trihydrate. 

Only telrapotassium diphosphate is of major industrial importance, due to its high water solubility. It is produced in an analogous way to the sodium compound from dipotassium hydrogen phosphate. 

Column uBondapak C18 (300x4 mm ID), mobile phase acetonitrile - methanol - phosphate buffer (0.038 M potassium dihydrogen phosphate, 0.005 M dipotassium hydrogen phosphate, pH 6.0) (16 5 79), flow rate 2 ml/min, detection UV 350 nm. Peaks 1, 3-demethylcolchicine 2, 2-de-methylcolchicine 3, N-desacetylcolchicine 4, 1-demethylcolchicine 5, N-methyl-N-desacetyl-colchicine 6, colchicine. 

The composition of the fungal medium used for the transformation studies was, sucrose(1.50 gm), glucose(1.50 gm), polypeptone(0.50 gm), dipotassium hydrogen phosphate(0.10 gm), magnesium sulfate(Q.50 gm), potassium chloride(0.50 gm) and ferrous sulfate heptahydrate(0.001 gm) in 100 ml of distilled water. 

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