Porcine LH

Matsuo H, Arimura A, Nair RMG, Schally AV. Synthesis of 78. the porcine LH- and FSH-releasing hormone by the solid-phase method. Biochem. Biophys. Res. Commun. 1971 45 822-827. 

Matsuo H, Baba Y, Nair RMG, et al. Structure of the porcine LH- and FSH-releasing hormone. I. The proposed amino acid sequence. Biochem Biophys Res Common 1971 43 1334-1339. 

Recently a fraction was recovered in each of the 5 fractionations of ovine SME with Sephadex which blocked the suckling-induced release of prolactin (Fig. 4). This fraction was separated from FSH, STH, MSH and TSH releasing factors and from vasopressin but was found to be closely associated with LH-RF as judged by the ovarian ascorbic acid depletion assay. However, in 3 of 5 experiments there was a tendency for PIF to be eluted just prior to the peak of LH-releasing activity. It is important to note that Schally et. al. (32) reported that LH-RF fractions from their Sephadex column were devoid of PIF when tested as to their ability to inhibit prolactin release in vitro. Arimura et. al. (33) found that purified porcine LH-RF was iivoTd of PIF activity as judged by its inability to block the release of prolactin in response to cervical probing in the rat. 

P-FSH Porcine pituitary extract Enriched FSH extract. Contains lower levels of LH and other pituitary proteins. Used to induce superovulatory response in animals... 

P-LH Porcine pituitary extract Used to induce ovulation in super-ovulated animals... 

Today s sequence techniques have achieved 9-10 orders of magnitude higher detection limit for PTH-amino acid than Edman s first report, in which he described the sequence of Ala-Leu-Gly with 100 pmol. Matsuo et al. succeeded in determing the structure of FSH-RH (LH-RH), a decapeptide hormone, in 1971 by using the Dansyl Edman method.83 This historical work used 200 nmol of FSH-RH purified from 165,000 porcine hypothalami and led a principal investigator, A. Schally, to win the Nobel Prize. Today, we would only need less than one hundred porcine hypothalami to complete the same job on a state-of-art sequencer. Those who plan the structural analysis of even barely detectable amounts of a peptide or protein are urged to start the plan such study could open up a new area in biochemistry. 

LH-RH 31, isolated in 1971 by Schally et al. 67) and in 1974 by Guillemin from porcine and sheep hypothalamus tissue, possesses LH-releasing and FSH-releasing activity and is available commercially as Lutal 68). 

The studies of the testis LH receptor indicated that it had a molecular weight of 194000 and that it exists as a dimer with both subunits having a molecular weight of 100000 [10-13]. In contrast, photoaffinity labelling [14,15] and chemical cross-linking [16] of LH with ovarian membranes suggests that the rat ovarian receptor consists of three or four subunits. It is possible that in addition to tissue differences there are species differences in the numbers and size of the subunits, because different results have been obtained from bovine [17] and porcine ovaries [18]. 

GnRH, which was originally known as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) releasing hormone, is a 10-residue polypeptide isolated from porcine hypothalami by Schally et al. (56) in 1971. It acts on the go-nadotrope to control the release of LH and FSH. 

Liu LH, Chen EC, Dorsey JL, Hsieh YH (2006). Sensitive monoclonal antihody-hased sandwich ELISA for the detection of porcine skeletal muscle in meat and feed products. J. Food Sci., 7LM1-M6. 

In order to assess the efficacy of electroporation in a model system that closely resembles human clinical use, the transdermal delivery of LHRH was studied using the isolated perfused porcine skin flap (IPPSF) model. The IPPSF model is a viable and vascularized in vitro system that was developed to quantify percutaneous absorption and cutaneous toxicity (Riviere et al, 1994 Riviere and Monteiro-ffiviere, 1991). The model has been used successfifily to predict human iontophoretic delivery ofarbutamine (Riviere et al, 1992b), to assess iontophoretic-induced skin irritation (Monteiro-Riviere, 1990), and to characterize the pathway of compound delivery across the skin (Monteiro-Riviere et al, 1994). Of particular relevance to the studies described here are our studies, in vivo and using the IPPSF model, of the iontophoretic deliveiy of LH (Heit et al., 1993, 1994). These studies have shown that LHRH is an excellent model peptide with which to... 

Follicle-Stimulating hormone-releasing hormone (FSH-RH). FSH-RH was discussed at this meeting by Dr. McCann and Dr. Jutisz. Our preparations of porcine and bovine FSH-RH seem to be considerably more potent than their preparations of ovine FSH-RH and are also free of LH> H. A complete separation of FSH-RH from LH-RH was achieved by preparative column electrophoresis (3, 18, 19). Thus fractions which release LH and have no effect on FSH secretion and conversely fractions which release FSH and have no effect on IM secretion. Highly purified FSH-RH at doses of 4-10 nanog depletes pituitary FSH content in castrated male rats pretreated with testosterone (3, 19). FSH-RH will also deplete pituitary FSH in normal male rats (3, 19). Some amines such as putrescine are also capable of depleting pituitary FSH content in vivo (20). Mittler and Meites (36) were the first to demonstrate the stimulatory effect of rat hypothalamic extracts on the release of FSH in vitro. e have shown by in vitro experiments that purified porcine FSH-RH stimulates the release of FSH by a direct action on pituitary tissue (21). Thus, addition of partially purified FSH-RH, but not of histamine, greatly enhanced the release of FSH. 

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