Porcine brain microvascular endothelial cells

II.I.l.l.l Primary Cultures of Porcine Brain Microvascular Endothelial Cells.. 522... 

Fischer S, Wiesnet M, Renz D, Schaper W (2005) H202 induces paracellular permeability of porcine brain-derived microvascular endothelial cells by activation of the p44/42 MAP kinase pathway. Eur J Cell Biol 84 687-697... 

Prior to transfection, the question of cell cytotoxicity needs to be addressed. Porcine microvascular endothelial cell (PBMEC) viability was affected in a concentration-dependent manner and was similar for linear PLL and cylindrical PLL brushes, with a 50% cell survival concentration of 0.5 mg/mL. Lor comparison, the cell cytotoxicity of linear PLLs and stiff cylindrical PLL-bmsh polymers on fibroblasts (N1H/3T3) was also tested, which is known to be very high for linear PLL [99]. These results could be confirmed because linear as well as brush PLL polymers were found to be very toxic for fibroblasts (50% cell survival concentration 0.01 mg/mL). The differences in cytotoxicity between the two cell lines may be explained by cell type-dependent differences. Brain endothelial cells possess transport systems (efflux transporter of ABC family) characteristic for barrier endothelial cells, which constitute a physical barrier to the brain [100]. The PLL-bmsh polymers showed a 40% reduction in cell viability at a concentration of 0.1 mg/mL and no effect at a concentration of 0.01 mg/mL. PLL bmsh concentrations used for transfection were <0.1 mg/mL hence, only moderate effects on cell viability are to be expected during transfection. Compared to the well-known cytotoxic side effects of other transfection reagents (e.g., lipofectins [101]), the moderate effects on cell viability observed here are judged to be of only minor importance. 

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