Porcine microvascular endothelial cell

Prior to transfection, the question of cell cytotoxicity needs to be addressed. Porcine microvascular endothelial cell (PBMEC) viability was affected in a concentration-dependent manner and was similar for linear PLL and cylindrical PLL brushes, with a 50% cell survival concentration of 0.5 mg/mL. Lor comparison, the cell cytotoxicity of linear PLLs and stiff cylindrical PLL-bmsh polymers on fibroblasts (N1H/3T3) was also tested, which is known to be very high for linear PLL [99]. These results could be confirmed because linear as well as brush PLL polymers were found to be very toxic for fibroblasts (50% cell survival concentration 0.01 mg/mL). The differences in cytotoxicity between the two cell lines may be explained by cell type-dependent differences. Brain endothelial cells possess transport systems (efflux transporter of ABC family) characteristic for barrier endothelial cells, which constitute a physical barrier to the brain [100]. The PLL-bmsh polymers showed a 40% reduction in cell viability at a concentration of 0.1 mg/mL and no effect at a concentration of 0.01 mg/mL. PLL bmsh concentrations used for transfection were <0.1 mg/mL hence, only moderate effects on cell viability are to be expected during transfection. Compared to the well-known cytotoxic side effects of other transfection reagents (e.g., lipofectins [101]), the moderate effects on cell viability observed here are judged to be of only minor importance. 

II.I.l.l.l Primary Cultures of Porcine Brain Microvascular Endothelial Cells.. 522... 

Fischer S, Wiesnet M, Renz D, Schaper W (2005) H202 induces paracellular permeability of porcine brain-derived microvascular endothelial cells by activation of the p44/42 MAP kinase pathway. Eur J Cell Biol 84 687-697... 

Deli et al. (2005) presented permeability data from various in vitro BBB models by measuring transendothe-lial electrical resistance (TEER) and by calculation of permeability coefficients for paracellular or transendothelial tracers. These authors summarized the results of primary cultures of cerebral microvascular endothelial cells or immortalized cell lines from bovine, human, porcine, and rodent origin. This also described the effect of coculture with astroglia, neurons, mesenchymal cells, blood cells, and conditioned media, as well as the physiological influence of serum components, hormones, growth factors, lipids, and lipoproteins on the BBB fimction. 

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