Polymerase chain reaction original

Figure 40-7. The polymerase chain reaction is used to amplify specific gene sequences. Double-stranded DNA is heated to separate it into individual strands. These bind two distinct primers that are directed at specific sequences on opposite strands and that define the segment to be amplified. DNA polymerase extends the primers in each direction and synthesizes two strands complementary to the original two. This cycle is repeated several times, giving an amplified product of defined length and sequence. Note that the two primers are present in excess.

Pollen analysis in combination with other techniques is still an effective tool for the authentication of the botanical origin of honey (Persano Oddo et ah, 1995 Von der Ohe et ah, 2004). It can distinguish polyfloral and different types of unifloral honeys (Mateo and Bosch-Reig, 1998). It can also indicate the percentages of different nectar contributions in honey products. A polymerase chain reaction technique and an... 

Mullis, K. (1990), The unusual origin of the polymerase chain reaction, Sci. Am. (April), 56-65. 

Mullis, K.B. (1990). The unusual origin of the polymerase chain reaction. Sci. Am. 262(4), 36-43. Narlikar, G.J. Herschlag, D. (1997). Mechanistic aspects of enzymatic catalysis Lessons from comparisons of RNA and protein enzymes. Annu. Rev. Biochem. 66,19-59. 

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