Polymerase chain reaction bacterial

Polymerase chain reaction (PCR DNA amplification of the most common bacterial meningitis pathogens) may be useful to help exclude bacterial meningitis. 

Polymerase chain reaction (PCR) is one of the most important techniques for rapid bacterial identification. It consists of repeated cycles of enzymatic reactions in a thermal cycler (PCR machine) that copies DNA strands many times. The DNA amplified in one PCR cycle is used as a template for the next cycle. This results in an exponential increase of the desired target... 

Hurst, G. Doktycz, M. Vass, A. Buchanan, M. Detection of bacterial DNA polymerase chain reaction products by matrix assisted laser desorption/ionization mass spectrometry. Rapid. Commun. Mass Spectrom. 1996,10,377-382. 

Additionally it has been our experience that mass spectrometry as a routine detection/identification technique for bacteria is not well received by microbiologists and clinicians who prefer less expensive, less complicated approaches to bacterial typing and identification, such as methods based on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA). For that reason we have adapted our MS approach to serve as a means of biomarker discovery that feeds candidate proteins or leads into development as PCR targets or other immunoassay techniques. 

Tsai, Y-L. Olson, B. H. (1992a). Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction. Applied and Environmental Microbiology, 58,754-7. 

There are several different methods based on 16S rRNA sequences that can be used to study bacterial community structure. The first approach involves extracting bulk nucleic acids from samples and then using this material in subsequent molecular analyses. A large number of these methods involve the polymerase chain reaction (PCR). Using PCR, target gene sequences... 

Assimilable organic carbon (AOC) was determined in water using flow-cytometric enumeration and a natural microbial consortium as inoculum.134 Two bacterial species were used for the measurement of AOC in water, based on their respective 16S rDNA sequences. The AOC content in 41 water samples was determined with these two sets by quantitative real-time polymerase chain reaction (qRT-PCR).135... 

The polymerase chain reaction (PCR) is an alternative to bacterial cloning. This is a method of gene amplification that does not require living cells it takes place in vitro and can produce (in a cost-effective way) commercial quantities of pure potential medicines. 

The polymerase chain reaction uses (1) a thermostable DNA polymerase, such as Taq polymerase derived from the bacterial thermophile Thermus aquaticus, (2) a DNA template which is to be amplified, (3) two primers, each typically of around 20 nucleotides, which anneal to distinct parts on the complementary strands of the target and serve as sites for commencing DNA polymerase action, (4) a solution including the four deoxynucleoside triphosphates dATP, dCTP, dGTP and dTTP, Mg2+, salts and pH buffer. 

Since then, many novel methods have been developed to analyze gene expression and mutation by using electrochemical techniques [1,2], matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MASS) [3], polymerase chain reaction (PCR) [4,5], bacterial magnetic particles [6], and microbeads [7]. 

Gelsomino, A., Keijzer-Wolters, A.C., Cacco, G., van Elsas, J.D. (1999). Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. J. Microbiol. Methods, 38, 1-15. 

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