Polymer streptavidin-conjugated

Smart polymer-streptavidin conjugates have been intensively studied by Hoffman and coworkers. An example is the preparation of copolymers based on acryhc acid and N-isopropylacrylamide (NIPAM) with high sensitivity to both pH and temperature, in a useful range of these properties. This copolymer is conjugated to a specific cysteine thiol site inserted by genetic... 

Fig. 16 Addition of 0.017-nmol aliquots of a rhodamine B-labeled streptavidin and b Texas Red-X-labeled streptavidin to 1.51 nmol of 43. Energy transfer observed in both cases with amplified emission of the dyes to the light-harvesting conjugated polymers. Direct excitation of the dyes at 575 and 585 nm correspond to 0.100 nmol of streptavidin.

PAP, peroxidase-antiperoxidase ABC, avidin-biotin conjugate APAAP, alkaline phosphatase antialkaline phosphatase B-SA, biotin-streptavidin PCNA, proiiferating cell nuclear antigen EPOS, enhanced polymer one-step staining (Dako) CARD, catalyzed reporter deposition HRP, horseradish peroxidase. 

A selective protein assay was demonstrated, which interfaces the FRET-based ratiometric techniques with the light-harvesting properties of conjugated polymer P4 [85]. Polymer P4 and a negatively charged biotinylated FI probe (Fl-B) were used to optically detect the target protein, Streptavidin. As shown... 

The fact that conjugated polymers are powerful tools in agglutination assays was first demonstrated by the exposure of biotinylated PPE 129 to avidin or streptavidin. Streptavidin binds to 129 and leads to agglutination and complete disappearance of the fluorescence in solution. Instead, a highly fluorescent pellet is formed. If 129 is treated with streptavidin-coated PS spheres, well-defined aggregates are formed. If similar biotinylated PPEs were treated with streptavidin, efficient energy transfer occurred when the streptavidin was labeled with Texas Red dye [127-129]. 

Bontempo, D., and H.D. Maynard. 2005. Streptavidin as a macroinitiator for polymerization in situ protein—polymer conjugate formation. Journal of the American Chemical Society 127 (18) 6508-6509. 

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