Polymer chip, fabrication

Sample Composition, TGA, and LOI Results of Compounded Polymer Chips, Tapes and Strings, and Effect of Additives on the Fiber and Fabric Properties... 

To overcome the above mentioned problems, we developed a self-assembly DNA-conjugated polymer for novel DNA chip fabrication [66-68]. The system developed uses ssDNA as a probe and disulfide bridges located in the polymer side chain for self-assembly immobilization. This polymer can be immobilized on gold substrate with the self-assembly technique. On the surface, DNA (hydrophilic) is exposed to a solution site without lying due to the hydrophobic polymer main chain (Fig. 4). Finally, we have discriminated differential of one base mismatched sequences using DNA-conjugated polymer. 

Using PAA as the starting point, a self-assembly DNA-conjugated polymer was prepared for DNA chip fabrication. The amounts of ssDNA and PDPH in the polymer were determined by absorption measurements to be equivalent to 1/714 [molecule/monomerunit] and 1/46 [molecule/monomerunit], respectively. A 20-mer ssDNA as P-1 DNA and PDPH for self-assembled immobilization were covalently attached to PAA as side chains. After self-assembled immobilization of the DNA-conjugated polymer on the gold surface of a sensor, the P-1 DNA chain was hybridized to a 34-mer ssDNA as P-2 DNA, which had a sequence fully matched to the desired target DNA. Analysis of the first hybridization (between P-1 and P-2 DNA) and of the second hybridization (between P-2 and the target DNA) was done by fluorescence measurements. 

A variety of techniques have been applied for sterilizing microfluidic cell culture chips and covered by reviews [12, 25] and references therein, such as autoclaving, UV light, oxygen plasma, gamma irradiation, ethylene oxide exposure and perfusion with ethanol, hypochlorite or sodium hydroxide. The applicability of the different techniques primarily depends, aside from what is available in a laboratory, on the type of system and the fabrication material. Autoclaving is an effective method but not suitable for chips fabricated of thermoplastic polymers. The applied temperature and pressure... 

The fabrication procedure for the two-tip device is basically the same as the single electrospray device. First, the two independent microchannels were hot embossed onto one cyclo-olefin polymer chip, and the two sample reservoir holes were drilled onto this cyclo-olefin chip at the end of the microchannels. Then, a gold electrode with a width of 500 pm was fabricated onto another cyclo-olefin chip. A hole for gold electrode access was drilled through the other... 

Polydimethylsiloxane (PDMS) is the commonly used polymer to fabricate microfluidic chips. PDMS is a biocompatible material with permeability to oxygen and carbon dioxide, thus favoring cell growth, apart from being optically transparent for easy observation. PDMS is prepared by mixing an elastomer and a curing agent in a specific ratio. The mixture is then poured over the mold and cured. Cured PDMS is then peeled from the mold and assembled to form the desired microfluidic platform. 

Sundararajan N, Kim D, Berlin AA (2005) Microfluidic operations using deformable polymer membranes fabricated by single layer soft lithography. Lab Chip 5 350-354... 

Fig. 7.13 Polymer cantilever fabrication process, (a) Evaporation of 5/50/50 nm of Cr/Au/Cr as release layer on a Si wafer, (b) Patterning of 1.5- am-thick SU-8 for the cantilever, (c) Evaporation of 5/60 nm Ti/Au for the resistors and wires, (d) Electroplating approximately 5 pm of Ni for the contact pads, (e) Patterning 3.5-pm-thick SU-8 for encapsulation of the resistors, (f) Patterning 200-pm-thick SU-8 for the microfluidic channel, (g) Release of the chip from the wafer by etching the Cr layer [Reprinted with permission from Johansson et al. (2005). Copyright 2005 Elsevier]...

G., and VoDertsen, F. (2005) UV-laser assisted fabrication of Bragg sensor components in a planar polymer chip. Sensor. Actuat. A. 120 (1), 44-52. 

In the experiments the p CD polymer film fabricated on a QCM chip has been characterized. The p CD polymer crosslinked by maleic acid (MA) was prepared in DMF in the presence of sodium hydride. The molar ratio of p CD, NaH and MA was 1 7 7. It was established that the primary and secondary hydroxyl groups of CD were substituted upon polymerization. 

GTP is a safe operation. A runaway polymerization can be quickly quenched with a protonic solvent. Since the group transfer polymerization goes to completion, no unwanted toxic monomer remains the silicone group on the living end after hydroxylation is removed as inactive siloxane. The living polymer in GTP is costlier than traditional polymerization techniques because of the stringent reaction conditions and requirements for pure and dry monomers and solvents. It can be used in fabrication of silicon chips, coating of optical fibers, etc. 

The application of polymer monoliths in 2D separations, however, is very attractive in that polymer-based packing materials can provide a high performance, chemically stable stationary phase, and better recovery of biological molecules, namely proteins and peptides, even in comparison with C18 phases on silica particles with wide mesopores (Tanaka et al., 1990). Microchip fabrication for 2D HPLC has been disclosed in a recent patent, based on polymer monoliths (Corso et al., 2003). This separation system consists of stacked separation blocks, namely, the first block for ion exchange (strong cation exchange) and the second block for reversed-phase separation. This layered separation chip device also contains an electrospray interface microfabricated on chip (a polymer monolith/... 

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