Polyinosinic polycytidylic acid Poly

A synthetic short double-stranded RNA such as Polyinosinic-polycytidylic acid (poly(I)-poly(C)) is widely used as a inducer in interferon production. The presence of DEAE-Dextran in the reaction medium increases the uptake of poly(I)-poly(C) by the cells and also makes the polynucleotide less susceptible to degradation from RNase. In addition, the... 

Polyinosinic polycytidylic acid (Poly I C) - a TLR3 agonist... 

Interferon is a low molecular weight protein, produced by vims-infected cells, that itself induces the formation of a second protein inhibiting the transcriphon of viral mRNA. Interferon is produced by the host cell in response to the vims particle, the viral nucleic and non-viral agents, including synthetic polynucleides such as polyinosinic acid polycytidylic acid (poly I C). There are two types of interferon. 

Synthetic polynucleotide complexes have been shown to be effective immune response modulators in animals and man (Braun et al. 1971, Johnson 1979). The polynucleotides are formed foUowing the action of an enzyme, polynucleotide phosphor-ylase on the synthetic mononucleotide diphosphates. Complexing takes place following the mixing of polymers composed of opposite base pairs. Two have been utilised, polyinosinic acid complexed with polycytidylic acid (poly I poly C) and polyad-enylic acid complexed with polyuridylic acid (poly A poly U). The single strands mononucleotides are ineffective. 

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