Polygalacturonases

Polygalacturonase activity in polyacrylamide gels (particularly after isoelectric focusing) can be detected by incubation of the gel with an appropriate substrate, after which it is stained with Ruthenium Red. Polygalacturonase isoenzymes were well separated from those of pectin lyase by isoelectric focusing. 

Polygalacturonases from an Aspergillus species and from Saccharomyces fragilis have been fractionated, and the products resulting from hydrolysis of pectate by the fractions were analysed by gel filtration at various stages of the reaction. Oligosaccharides were released by endo- and exo-polygalacturonases 

---

Tomato crtB from E. uredcvora Tomato polygalacturonase (PG) Increased total carotenoids Fraser et al., 2002... 

The most conspicuous concentrations of calciiun in the cell-walls of the flax hypocotyl were in the epidermal and subepidermal layers, especially at the tricellular junctions (figure 13 D), where these were filled with pectic polymers [67], Open tricellular jimctions with intercellular spaces had smaller areas of calcium accumulation where the walls of each pair of cells diverged. These sites were occupied by relatively linear pectic polymers with a low degree of esterification, which could be visualised with gold-kbeUed endopolygalacturonase [68] and were extractable by chelation of calcium with CDTA. Similar pectic polymers are located in the corresponding sites in other plant tissue, as established by susceptibility to polygalacturonase... 

Fig. 2 Inrnume complex binding enhancing activity of Carbohydrate fragments obtained by endo-polygalacturonase digestion...

The role of polygalacturonase, PGIP and pectin oligomers in fungal infection... 

The role of polygalacturonase in eliciting the plant defense responses... 

Kinetics and mode of action of AspergiUus niger polygalacturonases... 

Endo-polygalacturonases I and II (PGI and PGII) isolated from recombinant A. niger were characterized with respect to pH optimum, activity on polygalacturonic acid (pga), mode of action and kinetics on oligogalacturonates. 

Bussink et al. [4] and Kusters-van Someren et al. [5] have shown that in A. niger for both polygalacturonases (PGs) and pectin lyases (PLs) families of genes are present seven... 

Mode of action and kinetics. Routine polygalacturonase assays were performed in a reaction mixture containing 50 mM Na-acetate pH 4.2 and 0.25 % w/v pga at 30 °C. The release of reducing sugars was determined according to Stephens et al. [9]. For determination of pH optima the 50 mM Na-acetate buffer was replaced by Mcllvain buffers. 

Upon alignment of 25 endo- and exo-polygalacturonases taken from the swiss.prot. database only one histidine appeared to be conserved throughout. The conserved histidine at position 223 in PGII was changed into alanine, a small apolar residue. 

The hSubunit of Tomato Fruit Polygalacturonase Isoenzyme 1 Defines a New Class of Plant Cell Proteins Involved in Pectin Metabolism AroGPs (Aromatic Amino Acid Rich Glyco Proteins)... 

Figure 5. Polygalacturonase Isoenzyme Activities and Lycopene Production During Control 1 and TA8 Fruit Ripening.

Pressey, R. (1988). Reevaluation of the changes in polygalacturonases in tomatoes during ripening. Planta 174, 39-43. 

The Effect of Glycosylation of Eiutopolygalacturonases and Polygalacturonase Inhibiting Proteins on the Production of Oligogalacturonides... 

---