Polygalacturonase polymers

IRREVERSIBLE POLYMERIZATION POLYGALACTURONASE POLYMER FLEXING AND EXTENDING Polymeric substrate binding,... 

The most conspicuous concentrations of calciiun in the cell-walls of the flax hypocotyl were in the epidermal and subepidermal layers, especially at the tricellular junctions (figure 13 D), where these were filled with pectic polymers [67], Open tricellular jimctions with intercellular spaces had smaller areas of calcium accumulation where the walls of each pair of cells diverged. These sites were occupied by relatively linear pectic polymers with a low degree of esterification, which could be visualised with gold-kbeUed endopolygalacturonase [68] and were extractable by chelation of calcium with CDTA. Similar pectic polymers are located in the corresponding sites in other plant tissue, as established by susceptibility to polygalacturonase... 

In E. chrysanthemi, the pectate lyase, polygalacturonase and pectin methylesterase activities are induced in the presence of PGA (26, 37). The inducer is not the polymer itself but some breakdown products, initially generated by the... 

Preteatment with pectinases improves meehanieal debarking of wood significantly. In addition to polygalacturonase, enzymes hydrolysing the neutral polymers in cambial tissue (arabinanase, galactanase, and glucanase) are needed for effective solubilization of spruce cambium. 

Ishii (1981) has reported that a purified endo-polygalacturonase from Aspergillus japonicus solubilizes 95% of the total uronides from potato discs. After purification, three polymers were obtained of which the first two appeared to be Rhamnogalacturonans substituted with linear arabinans and galactans. The third fraction was highly branched and... 

Pereira, M., Wu, Y.-T., Venancio, A., Teixeira, J. (2003). Aqueous two-phase extraction using thermoseparating polymer a new system for the separation of endo-polygalacturonase. Biochemical Engineering Journal, 15, 131-138. 

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