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Exo-polygalacturonases

Upon alignment of 25 endo- and exo-polygalacturonases taken from the swiss.prot. database only one histidine appeared to be conserved throughout. The conserved histidine at position 223 in PGII was changed into alanine, a small apolar residue. [Pg.228]

While E. carotovora presents endo-polygalacturonase activity (10), the only hydrolase activity found in E. chrysanthemi is an exo-deaving polygalacturonase. Polygalacturonases hydrolyse a-l,4-glycosidic bonds they are active at addic pH (between 4 and 6.5) and do not require divalent cations. An exo-polygalacturonase... [Pg.317]

The right part contains less acidic and less charg polygalacturonases named A to J. All these fractions are enzymatically active, some have a true endo-polygalacturonase activity and others like fractions E juid I possess also an exo-polygalacturonase activity. [Pg.772]

The exo-polygalacturonase from Aspergillus tubingensis characterization and cloning of the gene... [Pg.817]

DC027 Suzuki, Y. Purification, and properties of carrot exo-polygalacturonase. Abstr... [Pg.211]

Mill (140, 141) isolated two exo-polygalacturonases from mycelial extracts of Aspergillus niger which diflFered in pH optima, stability, and activation by metal ions. Exopolygalacturonase I had a pH optimum of 4.5 and was fully active only in the presence of Hg ions. A ninefold increase in activity occurred in the presence of IfxM HgCb the Km was 6.2 X lO M. Dialysis against 2,3-dimercaptopropanol removed all activity in absence of Hg Various other divalent cations and chloride ion did... [Pg.114]

Omran, H., Buckenhueskes, H., Jackie, E., Gierschner, K. (1991). Einige Eigenschaften der Pectinesterase, der exo-Polygalacturonase und einer endo-6-1,4 Glucanase aus Einlege-gurken. Deutsche Lebensmittel-Rundschau, 87, 151-156. [Pg.538]

Polygalacturonases from an Aspergillus species and from Saccharomyces fragilis have been fractionated, and the products resulting from hydrolysis of pectate by the fractions were analysed by gel filtration at various stages of the reaction. Oligosaccharides were released by endo- and exo-polygalacturonases... [Pg.381]

See other pages where Exo-polygalacturonases is mentioned: [Pg.313]    [Pg.317]    [Pg.332]    [Pg.770]    [Pg.818]    [Pg.837]    [Pg.923]    [Pg.300]    [Pg.115]    [Pg.266]    [Pg.10]    [Pg.334]    [Pg.334]    [Pg.382]    [Pg.382]    [Pg.382]    [Pg.396]    [Pg.274]   
See also in sourсe #XX -- [ Pg.266 ]



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