Polyamidoamine efficiency

Haensler, J., and Szoka Jr., F.C., Polyamidoamine cascade polymers mediate efficient transfection of cells in culture, Bioconjugate Chemistry, 1993, 4, 372-379. 

Kukowska-Latallo JF, Bielinska AU, Johnson J, Spindler R, Tomalia DA, Baker JR Jr (1996) Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers. Proc Natl Acad Sci USA 93 4897 -902... 

As for polyethylenimines and polyamidoamines, PDMAEMA has been assessed for its transfection ability in vivo. Van de Wetering et al. (1999b) examined the efficiency of PDMAEMA in vivo and found that while ovarian carcinoma cells were able to be transfected in vitro and ex vivo at efficiencies up to 10%, they were not transfected in vivo. Components present in body fluids that are not present in the in vitro model may be responsible for the disappearance of transfection activity. This idea is supported by evidence that the presence of hyaluronic acid, one of many fluid components, greatly reduced in vitro transfection efficiency. 

Qin, L., Rahud, D.R., Ding, Y., Bielinska, A.U., Kukowska-Latallo, J.F., Baker, Jr., J.R. and Bromberg, J.S. (1998) Efficient transfer of genes into murine cardiac grafts by starburst polyamidoamine dendrimers. Hum. Gene Ther., 9, 553-560. 

Takahashi T, Harada A, Emi N, Kono K (2005) Preparation of efficient gene carriers using a polyamidoamine dendron-bearing lipid improvement of serum resistance. Bioconjug Chem 16 1160-1165... 

Several polycations with a good buffering capacity below physiological pH, such as lipopolyamines and polyamidoamine polymers, have proved to be efficient transfection agents (Boussif et al., 1995). Two of the most popular methods based on polycations are discussed below diethylami-noethyl-dextran (DEAE-dextran) and polyethylenimine (PEI). 

Kukowska-Latallo, J.E. Bielinska, A.U. Johnson, J. Spindler, R. Tomalia, D.A. Baker, J.R., Jr. Efficient transfer of genetic material into mammalian cells using starburst polyamidoamine dendrimers. Proc. Natl. Acad. Sci. USA 1996, 93, 4897-4902. 

An example for the use of fish embryo assays for the development of efficient nanosized PAMAM (polyamidoamine) dendrimers drug delivery tools is reported in Reference 39. PAMAM dendrimers with amino functional groups were toxic and attenuated growth and development of zebrafish embryos at... 

Kihara F, Arima H, Tsutsumi T, Hirayama F, Uekama K (2002) Effects of structure of polyamidoamine dendrimer on gene transfer efficiency of the dendrimer conjugate with a -cyclodextrin. Bioconjugate Chem 13 1211-1219... 

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