Plasma asparagine

Although asparaginase therapy has proved effective, a number of side-effects have been associated with initiation of therapy. These have included severe nausea, vomiting and diarrhoea, as well as compromised liver and kidney function. Side-effects are probably due to a transient asparaginase deficiency in various tissues. Under normal circumstances, dietary-derived plasma asparagine levels are sufficient to meet normal tissue demands, and the cellular... 

Example. A plethora of malignant tumors of need much more asparagine (a nonessential amino acid) in comparison to normal cells therefore, if by any manner the plasma asparagine gets destroyed (enzymatically), the cancerous tumor cells in turn are selectively starved to death. 

Fibrinogen (factor I, 340 kDa see Figures 51-1 and 51-4 and Tables 51-1 and 51-2) is a soluble plasma glycoprotein that consists of three nonidentical pairs of polypeptide chains (Aa,Bpy)2 covalently linked by disulfide bonds. The B(3 and y chains contain asparagine-linked complex oligosaccharides. All three... 

The cell wall of S. cerevisiae is composed of glucan, mannoproteins, and chitin (Klis, 1994 Cid et al., 1995). Of the mannoproteins, some of them are first synthesized as GPI-anchored and mannosylated proteins. Subsequently, they are incorporated from the plasma membrane to the cell wall and are covalently linked to the glucan there. Therefore, some signals should exist for dictating the cell wall incorporation. One found at a short N-terminal region near the GPI-attached asparagine (the co-site) is important (Hamada et al., 1998b). Namely, a plasma membrane GPI-anchored protein was localized to the cell wall if the (V/I)... 

The interassay variation was assessed in a plasma pool, which was stored at -20°C and analyzed 26 times over an 8-month period. The coefficients of variation (CVs) ranged from 6.1 to 8.9% with the following exceptions glutamine decreases steadily from 587 to 447 pmol/1 with a concomitant increase of glutamic acid from 62 to 164 pmol/1. Cystine decreased from 35 pmol/1 to undetectable. Tryptophan, arginine, methionine, asparagine, and proline had CVs slightly in excess of 10%. 

Mixed donor ligands. Thermodynamic parameters have been evaluated for the interaction of asparaginate with Fe2+ in aqueous solution at 25°C. On the basis of the results it is calculated that quite a large proportion of asparaginate in blood plasma may be complexed to iron(u) as well as to the expected copper(n).93 1.10-Phenanthro-line /V-oxide (phenNO) forms the complex [Fe(phenN0)3](C104)2 in which the ligands chelate by N- and O-donor atoms.96... 

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