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Photoaffinity labels properties

Aryl nitrenes have been studied extensively over the last decades and are used in several industrial processes such as microlithography. Aryl nitrenes have also been used in photoaffinity labeling bioorganic molecules. The pursuit for organic magnetic material has sparked renewed interested in nitrene intermediates, which are ideal candidates for magnetic material because of their high spin properties. ... [Pg.409]

Galardy et al. (1973) introduced acetophenones and benzophenones for photoaffinity labeling (see also Martyr and Benisek, 1973 Katzenellenbo-gen et al., 1974). The properties of excited states generated from these and other a,P-unsaturated ketones are well understood (Turro, 1979). In most cases a triplet excited state is formed that abstracts a hydrogen atom from a donor yielding two radicals which subsequently couple. Unreacted excited species relax to the ground state and may be excited repeatedly until they react (Fig. 2.5). [Pg.15]

Herbicides that inhibit photosynthetic electron flow prevent reduction of plastoquinone by the photosystem II acceptor complex. The properties of the photosystem II herbicide receptor proteins have been investigated by binding and displacement studies with radiolabeled herbicides. The herbicide receptor proteins have been identified with herbicide-derived photoaffinity labels. Herbicides, similar in their mode of action to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) bind to a 34 kDa protein, whereas phenolic herbicides bind to the 43-51 kDa photosystem II reaction center proteins. At these receptor proteins, plastoquinone/herbicide interactions and plastoquinone binding sites have been studied, the latter by means of a plastoquinone-deriv-ed photoaffinity label. For the 34 kDa herbicide binding protein, whose amino acid sequence is known, herbicide and plastoquinone binding are discussed at the molecular level. [Pg.19]

In the course of studies directed towards the development of photoaffinity labels for monoamine oxidase (MAO) isozymes, Shih and colleagues found that 4-fluoro-3-nitrophenyl azide (FNPA) (16) exhibited some interesting properties as an inhibitor of this enzyme [80]. MAO catalyzes the oxidative... [Pg.141]

Studies of the photodecomposition of azido derivatives of PCB have attracted interest because of their potential use as photolabels in intracellular distribution studies.Polyfluorinated aryl azides are also of current interest as new reagents for photoaffinity labelling. The major products of photolysis of methyl 4-azidotetrafluorobenzoate in cyclohexane or diethylamine, for example, arise by insertion, a property which enhances its use in labelling. Singlet pentafluorophenyl nitrene, formed in an analogous fashion on irradiation of pentafluorophenyl azide, can be trapped by toluene as the insertion products (79) and (80). °... [Pg.381]

Esters and thioesters of (dansyldiazomethyl)methylphosphinic acid undergo carbene insertion reactions in high yield on irradiation their fluorescent properties make them suitable reagents for photoaffinity labelling studies. [Pg.484]

Derivatives of Cgo for photoaffinity labelling studies have been synthesized, including the cis and treats diazirine compounds (21a). The photochemical properties of these are currently being studied in various applications. An... [Pg.209]

Our knowledge about supramolecular nitrene chemistry and the reactivity of these intermediates in a constrained system is still in its infancy even though nitrenes are widely used for photoaffinity labelling. However, the exact structures of the products formed after reaction with the active sites are often unknown. Therefore, a better understanding of the binding properties of a nitrene precursor within the host molecule is necessary. Moreover, it is essential to learn which reactions still do occur inside a supramolecular structure. [Pg.295]

Use of 3(3-azido-4 chlorophenyl)-1,1-dimethylurea (azidomonuron), a photoaffinity labelling analogue of the herbicidal phenylurea 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), has revealed Tyr 237 and Tyr 254 of D1 to be close to the herbicide binding site (1). An interesting property of DCMU is its ability, under certain conditions, to displace a bicarbonate ion which normally binds to PS2 in the region of the non-heme ferrous atom situated between and Qg. [Pg.603]

Goeldner, Hirth and colleagues have presented evidence that the efficiency of labeling by certain photoaffinity reagents is improved in the environment provided by the receptor. They define a photosuicide inhibitor as a ligand analog of an enzyme or a receptor, the photodecomposition of which is selectively induced by the intrinsic physico-chemical properties of an active site (Goeldner et al., 1982). [Pg.23]

The distinctly different behavior of the phenol-type herbicides following trypsin treatment suggests that different determinants within the PS II protein complex establish the "domains" that regulate the binding properties of these inhibitors. In spite of the fact that phenol-type herbicides will displace bound radiolabeled herbicides such as diuron, these inhibitors show noncompetitive inhibition (29, 30). At present, there are three lines of evidence which favor TH e involvement of two domains within the PS II complex that participate in creating the binding sites for these herbicides (a) isolated PS II particles can be selectively depleted of a polypeptide with parallel loss of atrazine sensitivity, but not dinoseb inhibition activity (33) (b) in resistant weed biotypes, chloroplast membranes that exhibit extreme triazine resistance have increased sensitivity to the phenol-type herbicides (13) and (c) experiments with azido (photoaffinity) derivatives of phenol and triazine herbicides result in the covalent labeling of different PS II polypeptides (, 31). [Pg.43]


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See also in sourсe #XX -- [ Pg.24 , Pg.26 ]




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