Phosphorylcholine inhibition

Harnett, W. and Harnett, M.M. (1993) Inhibition of murine B cell proliferation and down-regulation of protein kinase C levels by a phosphorylcholine-containing filarial excretory-secretory product. Journal of Immunology 151, 4829-4837. 

Mitchell, G.F. and Lewers, H.M. (1976) Studies on immune responses to parasite antigens in mice IV. Inhibition of an anti-DNP antibody response with antigen, DNP-Ficoll containing phosphorylcholine. International Archives of Allergy and Applied Immunology 52, 235—240. 

To suppress cell adhesion on a material surface, one approach is to inhibit the adsorption of proteins. SAMs of alkanethiols that carry oligo(ethylene glycol) (OEG) [69] and phosphorylcholine [46, 70, 71] have been shown to prevent... 

Diacylglycerol has long been known to be a weak competitive inhibitor of PLC/fc, whereas phosphorylcholine shows very little inhibition [40, 49, 116]. Recent kinetic assays of PLCB(. activity in the presence of DAG indicate that it is a competitive inhibitor with a Kl of the order of 10 mM, whereas phosphorylcholine was found to be an extremely weak (K = 30-50 mM), mixed inhibitor of PLC/J( [34]. Because diacylglycerol is a competitive inhibitor of the enzyme, the nature of the catalytic cycle dictates that it must be the last product to leave the enzyme active site. 

In parallel to catheter-based delivery, stent-based approaches, such as passive stent coatings (diamond-like carbon, phosphorylcholine, and silicon carbide coatings) and immobilized drug coatings (heparin-coated stents), were evaluated for their ability to inhibit restenosis. Although animal studies demonstrated some promise, none of these technologies were clinically successful for restenosis prevention. The failure of these surface modification technologies further added to the need for the development of DES based on the principles of sustained CDD,... 

A thorough study of phosphorylcholine CTP cytidyltransferase in green onions (Allium cepa L.) was made by Morre et al. (1970). They found most of the activity in the 100,000-g particulate fraction but significant activity in the supernatant. The two fractions behaved similarly with respect to saturation by phosphorylcholine, activation by Mg + and Mn ", inhibition by Ca +,... 

Cosenza and Kohler (47,48) and Sher and Cohn (49) found that antiidiotypic antibodies to the myeloma proteins, TEPC 15 or SI07, which have antipneumococcal (antiphosphorylcholine) activity, almost completely inhibit plaque formation by spleen cells from BALB/c mice immunized with heat-killed strain R36A pneumococci. Erythrocytes used in the plaque-forming tests could be coated either with pneumococcal C-polysaccharide or with phosphorylcholine, with similar results. These significant experiments indicated that nearly all the antibodies produced by BALB/c mice in the primary in vitro response to the pneumococcal polysaccharide shju-e idiotype with a group of mouse... 

The response of blomolecules and cell membranes Is determined by many factors, some of which are the chemical composition and conformation of the molecules, the surface energy, and topography of the top surface layers which are in contact with biological systems, I.e., body fluids and cells [45]. The work illustrated here consisted In designing new polymers with functional properties capable of promoting the attachment of specific cells. The first step consisted in a polymer system which surface inhibits non-specific cell attachment. This strategy is based on the incorporation of cell membrane constituents such as phosphorylcholine (PC) or phospholipid analogues into polymers [46-51]. 

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