Phage induction tests

Mutagenesis tests of 2,6-xylidine have proved equivocal. o-Toluidine has displayed positive results in DNA repair assays and phage induction assays. The parent compounds displayed no mutagenicity. The mutagenesis potential has not been evaluated in the majority of local anesthetics. 

Kada T, Green M, Mandel M, et al. 1981. Summary report on the performance of bacterial repair, phage induction, degranulation, and nuclear enlargement assay. In deSerres FJ, Ashby J, eds. Evaluation of short-term tests for carcinogens Report of the International Collaborative Program. Prog Mutat Res 1 33-48. 

Catalytic antibodies, like enzymes, must be isolated and purified to homogeneity before they can be studied. Initially this was done by using the hybridoma technique for isolation of monoclonal antibodies (Box 31-A). After induction of antibody formation by injecting a selected hapten into a mouse, large numbers of monoclonal antibodies had to be tested for catalytic activity. Even if several thousand different monoclonal antibodies were tested, only a few with catalytic properties could be found.1 Newer methods have incorporated recombinant DNA techniques (Box 31-A) and use of combinatorial libraries and phage display.) Incorporation of acidic or basic groups into the haptens used to induce antibody formation may yield antibodies capable of mimicking the acid-base catalysis employed by natural enzymes. 0... 

Heinemann B, Howard AJ. 1964. Induction of lambda-bacterial phage in Escherichia coli as a screening test for potential antitumor agents. Appl Microbiol 12 234-239. 

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