Phage display receptors

Phage display of random peptide libraries identified agonists of erythropoietin receptor... 

Protein engineering is now routinely used to modify protein molecules either via site-directed mutagenesis or by combinatorial methods. Factors that are Important for the stability of proteins have been studied, such as stabilization of a helices and reducing the number of conformations in the unfolded state. Combinatorial methods produce a large number of random mutants from which those with the desired properties are selected in vitro using phage display. Specific enzyme inhibitors, increased enzymatic activity and agonists of receptor molecules are examples of successful use of this method. 

Jacobsson, K., and Frykberg, L. (1995). Cloning of ligand-binding domains of bacterial receptors by phage display. BioTechniques 18, 878-885. 

Sche, P. P., McKenzie, K. M., White, J. D., and Austin, D. J. (1999). Display cloning functional identification of natural product receptors using cDNA-phage display. Chem. Biol. 6, 707-716. 

Rajotte D, Ruoslahti E. Membrane dipeptidase is the receptor for a lung-targeting peptide identified by in vivo phage display. J Biol Chem 1999 274 11593-11598. 

Phage displayed peptide libraries have been snccessfnlly employed for the identification of peptide ligands with a variety of applications. Recent reviews describe the developments in the generation and screening of peptide libraries [6], their application in the identification of receptor ligands [32] and in drng development [33]. 

The use of phage-display techniques has identified peptide ligands with specific affinity to cell surface receptors or specific tissues (see Table 11.2). Such peptide homing devices can be... 

Peptide Receptor Ligands from Phage Display Peptide Library. 269... 

There has been substantial progress in the construction of phage-displayed peptide libraries and in screening methods with the libraries to isolate peptide receptor ligands [66,116-119]. Phage display peptide libraries offer opportunities to characterize peptide binding specificity of important proteins, such as... 

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