Perspex lid

Figure 23. The gel electrofocusing apparatus used by Leaback and Rutter (78). It comprises a perspex lid bearing terminals connected to horizontal carbon or platinum electrodes (E), and a glass base to carry the gel (G) and the electrode wells (A and B). The base consists of a glass plate to which 4 strips of ass are bonded (with Araldite epoxy-resin) to form a watertight well 18 X 8 X 0.2 cm, a perspex strip 8 X 2 X 0.2 cm displaced at (A) and another at (B) before the well is filled with polymerization mixture. Electrode wells (A) and (B) are filled with aqueous solution of phosphoric acid and diaminoethane respectively. The samples are added to slots (S) and with the lid in position, a potential difference is applied to the electrodes with the current kept at or below 2 mA. A moist pad (C) prevents excessive evaporation from the gel surface. (Leaback and Rutter, 37.)...

Humidified staining box either an adapted sandwich box or purpose-made black perspex box with transparent perspex lid (Genex Ltd., Coolsdon, Surrey, UK). Blocking solution, either bovine serum albumin (BS A) (0.1-1%), or normal serum (1-5%) from the species in which the secondary antibody was raised, diluted in PBS. 

Phenylacetylene. Support a 5-litre glass Dewar flask in a wooden case. Equip the flask with a lid of clear Perspex, provided with suitable apertures for a mechanical stirrer, introducing solids (e.g., sodium) or hquids, a calibrated dip stick for measuring the volume of liquid in the Dewar vessel, a gas mlet tube and an ammonia inlet arrange for an electric light to shine downwards into the flask. 

Place in a humid incubator at 37°C with an atmosphere of 10% C02, 5% 02 and air. If a low oxygen incubator is not available, gas cylinders containing the C02/02 mixture may be rented. In this case a perforated sheet of perspex can be laid on top of four silicone bungs ( 3 cm long) and sterile water added to the box. Place the dishes on top of the tray and replace the lid, which should have a hole drilled in it. Seal the lid to the box with insulation tape and gas the box through the hole in the lid for 10 min. Seal the hole in the lid with tape and place the box at 37°C. 

In vessel (2) there is a perspex cone (11) the top of which forms a lid (10) to vessel (2). This cone changes the density gradient so that it w ill not be exponential but linear. A holder (12), with a slit and wing screw (18), is mounted on the bracket to enable the gradient mixer to be fastened to the stand. 

The separation cell (Figures 32 and 33) is made of Perspex with a wall thickness of 1 mm. It resembles a series of Tiselius cells with a cross section of 3 X 40 mm. Each U-tube unit is about 10 mm high. The channels are spaced 10 mm apart. The cell con ts of two separate parts, the trough and the lid. The trough is a shallow rectangular box with a... 

The materials of construction of the vessel or pipe and all internals (impeller or mixers, shaft, baffles, bearings, seals, and lid) must be compatible with the fluids and any solids that are to be mixed. For most mixing experiments the ability to see clearly what is happening inside the mixer is exfiemely useful, if not essential. Vessels or pipes made of a transparent material such as polymethylmethacrylate (PMMA Perspex or Lucite) or glass are ideal, provided that measurements are to be made at temperatures and pressures within the modest limits allowed by such materials (the glue used to seal PMMA vessels tends to fail at about 50°C). PMMA vessels up to about 1 m in diameter can be manufactured relatively simply. 

The apparatus consists of a perspex box 15 X 25 X 60 cm with a gabled lid, so that liquid condensing on the top does not drip on to the chromatograms. The walls of the box are lined with cotton cloth, over which both phases are pumped continuously by a diaphragm pump delivering 1,500 ml per minute. The liquids are drawn from the bottom of the box, and, in setting up the apparatus, special attention is paid to ensuring that both phases are circulated. 

An enclosiu e for spray ejected into ambient conditions is shown in Fig. 16.3. It is used to analyze the effect of entrainment on the resulting spray. Entrainment of air occurs since liquid, exiting the nozzle, accelerates the surrounding gas which is then mixed into the spray. By applying the shown enclosure, entrainment is lowered due to the lid-like structure that hampers air flow in the near nozzle region. In order to allow a visualization of the spray inside the enclosure, it is made of Perspex. In the middle of the enclosure, there is an inlet for the atomizer, which is connected to the test facility described in Fig. 16.1. The enclosure is open at the bottom. 

The interface of the leachate and clay liner material was modelled using a simple tank system. A perspex box (350 x 350 x 50 mm) was filled with a layer (depth 20 mm) of pre-saturated clay. The clay was collected at the same time and from the same location as the samples used in the column experiments and was pre-saturated with tap water, to minimize wetting and capillary effects. The tank was filled with leachate to a depth of 40 mm and the head space flushed with nitrogen. An oversized lid and water filled trench... 

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