Mammalian cell extracts

Gill, D.M., Pappenheimer Jr, A.M, Brown, R., and Kurnick, J.T. (1969) Studies on the mode of action of diphtheria toxin VII. Toxin-stimulated hydrolysis of nicotinimide adenine dinucleotide in mammalian cell extracts./. Exp. Med. 129, 1-21. 

Podlutsky AJ, Dianova, n, Wilson SH, Bohr VA, Dianov GL. DNA synthesis and dRPase activities of polymerase beta are both essential for single-nucleotide patch base excision repair in mammalian cell extracts. Biochemistry 2001 40 809-813. 

Mu D, Bessho T, Nechev LV, Chen DJ, Harris TM, Hearst JE, Sancar A. DNA interstrand cross-hnks induce futile repair synthesis in mammalian cell extracts. Mol. Cell. Biol. 2000 20(7) 2446-2454. 

Liu B, Han Y, Ferdous A, Corey DR, Kodadek T. Transcription activation by a PNA-peptide chimera in a mammalian cell extract. Chem Biol. 2003 10 909-916. 

Dianov GL, Bischoff C, Piotrowski J, Bohr VA (1998) Repair pathways for processing of 8-oxoguanine in DNA by mammalian cell extract J Biol Chem 273 33811-6 Fortini P, Pascucci B, Parlanti E, Sobol RW, Wilson SH, Dogliotti E (1998) Different DNA polymerases are involved in the short- and long-patch base excision repair in mammalian. Biochemistry 37 3575-80... 

Two classes of proteins ( 28 kilodaltons and 80-100 kilodaltons) have been identified in mammalian cell extracts that bind specifically to the two major types of cisplatin-induced DNA 1,2-intrastrand crosslinks. The proteins do not bind to adducts produced by the inactive transplatin. A gene that encodes one protein of molecular weight of approximately 81 kilodaltons (termed struc-... 

Shibutani, S. and Grollman, A.P. 1994. Miscoding during DNA synthesis on damaged DNA templates catalysed by mammalian cell extract. Cancer Lett. 83, 315-322. 

Allinson, S. L., Dianova, I. I., and Dianov, G. L. (2001). DNA polymerase beta is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO J. 20, 6919-6926. 

These studies used randomly damaged DNA and could not distinguish between inhibition of repair in the nucleosome core or linker DNA. More recent studies have used the in vitro assembly of nucleosomes containing site-specific DNA lesions within the core particle and either purified repair factors or mammalian cell extracts to examine in more detail the effect of chromatin structure on excision repair. It was determined that UV-induced photolesions as well as AAF- and cisplatin-modified bases located in nucleosome cores are repaired at a 5- to 10-fold reduced rate relative to the same lesions within the same sequence context in naked DNA (Hara and Sancar, 2002, 2003 Hara et al., 2000 Wang et aL, 2003). 

Sorensen, M. S., Duch, M., Paludan, K., Jorgensen, P., and Pedersen, F. S. (1992) Measurement of hygromycin B phosphotransferase activity in crude mammalian cell extracts by a simple dot-blot assay. Gene 112, 257-260. 

Fig. 9 Normalised binding data obtained for purified human p52 DBD, bacterial cell extracts containing p52 DBD and mammalian cell extracts containing over-expressed full-length p52 on Codelink protein-DNA microarrays. The sequence GGGGTTCCCC (NF-kB14) was assigned a value of 1000 in each experiment. NF-kB18, NF-kB21 and NF-kB28 are classified as medium binders (Fig. 7) NF-kB30 is a low binder...

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