PEI cellulose

ECTEOLA-Cellulose = Epichlorhydrin linked triethanolamine cellulose, and PEI-Cellulose = Polyethylenimine cellulose. 

Use a sheet of polyethyleneimine cellulose (PEI Cellulose), 50 x 200 mm, for chromatography. Mark the start about 15 mm away of the shorter side with a pencil. Apply the sample with a pipet or syringe. If larger volumes have to be used, apply them in several portions and/or a short line. 

Mark the start with a pencil on a PEI Cellulose sheet (dimensions as in Protocol 3.1.2) and apply the samples with a microliter syringe or a pipet. 

A variety of powerful methods is available for fractionating short oligonucleotides. Work on nucleotide sequence analysis of small RNA molecules (n=80 or 120) and more recently viral RNA molecules (n= 3000) and even ribosomal precursor RNAs (up to 45 s) (Holley et al. 1965a Brownlee 1972 Maden et al. 1972) has stimulated the development of these procedures. They can also be used for synthetic oligonucleotides (e.g. Hachman and Khorana, 1969). The methods depend largely on chromatography and electrophoresis on filter papers, diethylaminoethyl(DEAE) paper, cellulose acetate, thin layers of cellulose or polyethyleneimine (PEI)-cellulose, or columns. 

Randerath and Randerath (1967a) have developed a thin layer method specific for nucleotides using PEI-cellulose (polyethyl-... 

Several functional groups have been used to obtain cellulose anion exchangers [aminoethyl (AE), diethyl-aminoethyl (DEAE)], or cation exchangers [car-boxymethyl (CM), phosphate (P)] for thin-layer chromatography. PEI cellulose is not a chemically modified cellulose, but a complex of cellulose with polyethyleneimine. These cellulose exchangers are particularly useful for the separation of proteins, aminoacids, enzymes, nucleobases, nucleosides, nucleotides, and nucleic acids. 

PEI-Cellulose Ion exchange Nucleic acid Dil. HCl (0.01-0.1 M) Autoradiography Also two-way development... 

The final compounds are then isolated by any of the fast selective procedures available. These include thin-layer chromatography (tic) on polyethyleneimine (PEI) cellulose plates, slab preparative gel electrophoresis, and hplc on Per-maphase AAX or Partisil lOSax column. 

Several chromatographic methods exist for the analytical study of sugar nucleotides. These include the ethanol-ammonium acetate systems above and ion-exchange on polyethylene-imine paper (PEI paper) or, better, on PEI-cellulose and ECTEOLA cellulose layers. Paper and thin-layer electrophoresis is also useful, provided that extremes of pH are avoided. As a general... 

An ATPase assay is performed by monitoring ADP formation using [ot- P] ATP (400 Ci/mmol, Amersham Pharmacia Biotech) on polyethyleneimine (PEI) cellulose thin-layer sheets (TLC sheet, Polygram cel 300 PEI, Macherey-Nagel, Germany), based on the procedure reported previously. ... 

Spot aliquots (2 jl1) on PEI cellulose thin-layer sheets and dry. Develop with 1 M LiCl by one-dimensional chromatography. 

Fig. 2A-D. Effect of RNase A on the average chain length of poly(ADPR) syn-thetized by the mRNP poly(ADPR) polymerase. The reaction was carried out as described in [4] with addition of variable amounts of RNase A A control B 1.5 jug ml" C 5 Mg ml" D 20 Mg ml". The reaction product was analyzed by thin layer chromatography on PEI cellulose after digestion by snake venom phosphodiesterase. Average chain length A 3.9, B4.2,C4.5,D4.7...

Inosine monophosphate (IMP) can be detected in nucleotides following TLC purification. In the first stage, the sample is introduced into a mixture of water (10 p,l) and 100 mM non-radioactive 5 IMP (1 p,l) 2.5 p,l from this mixture is spotted onto the chromatographic plate at 2.5 cm from the side of the plate. After drying of the spots, the plate is introduced into a methanol atmosphere (10 min), dried, and then bidimensionally developed on polyethyleneimide (PEI)-cellulose. 

The nucleotides were separated on PEI-cellulose in 0.9 M guanidinium chloride. After separation, the plate was introduced, for 10 min, into absolute methanol and then dried in air. The plate was irradiated in a nuclear reactor for 96 hr, with a 4 X lO neutrons/cm/sec flux. The nucleotides with phosphorus ( P) are activated at... 

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