ECTEOLA-cellulose

Purified by Sephadex G-200 filtration and DEAE-cellulose column chromatography. Hexosaminidase A was further purified by DEAE-cellulose column chromatography, followed by an ECTEOLA-cellulose column, Sephadex-200 filtration, electrofocusing and Sephadex G-200 filtration. Hexosaminidase B was purified by a CM-cellulose column, electrofocusing and Sephadex G-200 filtration. [Srivastava et al. 7 Biol Chem 249 2034 1974.]... 

ECTEOLA-Cellulose = Epichlorhydrin linked triethanolamine cellulose, and PEI-Cellulose = Polyethylenimine cellulose. 

CM cellulose (carboxymethyl) CM 22, 23 cellulose P cellulose (phosphate) SE cellulose (sulphoethyl) SM cellulose (sulphomethyl) DEAE cellulose (diethylaminoethyl) DE 22, 23 cellulose PAB cellulose (p-aminobenzyl) TEAE cellulose (triethylaminoethyl) ECTEOLA cellulose... 

Ecteola Cellulose treated with ethanolamine and epichlorhydrin... 

DEAE cellulose (diethylaminoethyl) DE 22, 23 cellulose PAB cellulose (p-aminobenzyl) TEAE cellulose (triethylaminoethyl) ECTEOLA cellulose... 

ECTEOLA-cellulose. (epichlorohydrin triethanolamine cellulose). A dry, powdered cellulose derivative containing tertiary amine groups. 

The second calcium-binding protein present in the soluble extract of pig brain is eluted from ECTEOLA cellulose by 50 M Tris HC1 />H 7.4 containing 0.32 M NaCl (159). This protein will be designated brain CBP-II for the sake of clarity of this discussion. The ECTEOLA-cellulose fraction containing this protein was incubated with 45CaCl2 and subjected to gel filtration on Sephadex G-75. A single peak of protein-bound radioactivity was found, and no free calcium peak was detected (Fig. 4). 

Fig. 4. Gel filtration (Sephadex G-75) of the soluble proteins from pig brain which are eluted from ECTEOLA cellulose at a NaCl concentration between 0.1 M and 1 M. The proteins in this fraction were concentrated in a Diaflo apparatus and incubated with 25 fid 45CaCl2 before gel filtration. Absorbancy at 750 M following Lowry reaction (—) radioactivity (---). From Ref. (159)...

ECTEOLA-Cellulose. Cellulose-ECTEOLA. Anior,-exchange material prepd from cellulose, epichlorohy-drin, and triethanolamine Peterson. Sober, / Am. Chem. Soe. 78, 751 (1956) Veder. J Chromatog. 10, 507 (1963). 

Several chromatographic methods exist for the analytical study of sugar nucleotides. These include the ethanol-ammonium acetate systems above and ion-exchange on polyethylene-imine paper (PEI paper) or, better, on PEI-cellulose and ECTEOLA cellulose layers. Paper and thin-layer electrophoresis is also useful, provided that extremes of pH are avoided. As a general... 

The interactions of cellulose, cellulose triacetate, carboxymethyl-, phosphato-, DEAE-, trimethylaminoethyl-,and ECTEOLA-celluloses withaflatoxins Bj and Gj have been studied by means of equilibrium dialysis. ... 

DEAE-Cellulose, ECTEOLA-Cellulose, Carboxymethyl-cellulose,Phosphoryl-cellulose cf. pp. 39, 43, 45, 47 in [124]. 

Ion exchange-layers like DEAE- and ECTEOLA-cellulose [11, 35,59, 78] and PP-cellulose [55] also are suitable for separating bases and nucleosides. 

Water has been used as solvent on DEAE- and ECTEOLA-cellulose layers [11, 59] and also 0.005N hydrochloric acid [11] and isobutyric acid-conc. ammonium hydroxide-water (66 + 2 + 32) on the former [11]. Saturated ammonium sulphate solution-N sodium acetate-isopropanol (80 -f 18 + 2), a solvent which was developed for the PC [45] and TLC (cellulose) [60] of nucleotides, is particularly good for separating purine and pyrimidine bases on layers of DEAE-cellulose [11]. 

Table 193 gives a comparison of the separations of nucleobases and nucleosides on paper and on layers of cellulose, silica gel G and ECTEOLA-cellulose. The extensive agreement of the hJK/-values of the purine bases and purine nucleosides on the cellulose and ECTEOLA-ceUulose layers and on paper, is striking appreciably higher LB/-values were found on silica gel G. The various pyrimidines and pyrimidine-ribonucleosides have very nearly identical LB/-values on the three layers and on paper. A better separation of the purine and pyrimidine bases from the corresponding ribonucleosides is achived on cellulose and ECTEOLA cellulose layers than on sihca gel G. These three adsorbents are about equally useful for fractionating the nucleosides. 

Hydrochloric acid (0.02—0.04 N) is suitable for fractionating mononucleotides on layers of DEAE- and ECTEOLA-cellulose [26, 66] (see also [104]) the runs are rather longer on the former. The mononucleotides can be separated on PEI-cellulose by stepwise development using N acetic acid and 0.1—0.4N sodium chloride or lithium chloride [57, 70]. Complex mixtures of these compounds may be fractionated by two-dimensional TLC on PEI-cellulose stepwise development is carried out in the first direction with 0.2M, IM and 1.6M lithium chloride the lithium salt is then washed out of the layer by laying the chromatogram for 15 min in a flat dish containing 0.5-1 litre absolute methanol finally, stepwise development is performed in the second direction using 0.5M, 2M and 4M sodium formate buffer, pH 3.4 [56] (Fig. 222). 

Aqueous 0.15M sodium chloride solution is convenient for fractionating mono-, di- and triphosphates on DEAE- and ECTEOLA-cellulose [59]. 0.3—0.5M lithium chloride may be used in TLC on PEI-cellulose of these compounds as well as of pyridine-nucleotides the separations do not take longer than 15 min [51, 63, 70, 75]. 0.3 0.8M ammonium sulphate is also suitable for fractionation of mono-, di- and triphosphates [77]. 

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