Passive transfer

As will be described in detail below, solute distribution in biphasic systems can be modulated by application of a Galvani potential difference across the interface, thereby leading to the transfer of species from one phase to the other. Therefore, in electrochemical terms, passive transfer simply means the partition across an interface, mediated by a potential-driven process. 

AP immunotherapy has been used in both prevention and treatment trials in mutant mice. Both Ap immunization (with Freund s adjuvant) and passive transfer of Ap antibodies reduce levels of AP and plaque burden in... 

It now appears certain that destruction of malaria parasites in vivo depends in part, if not wholly, upon the presence of humoral antibodies, and this must explain the success achieved with the passive transfer experiments. The exact role of the T and B lymphocytes in immunity in malaria infection in man still needs clarification. In rats the ability to resist P. berghei infection seems to be thymus dependent, and higher parasitemias were encountered in the thymectomized rats, which also developed severe anemia (B6). 

Increasing permeability and decreasing turnover will result in the CSF concentration of albumin approaching its serum concentration because of the passive transfer of albumin through the barrier (FI). Identical concentrations (R= 1) are not quite reached, but the albumin ratio can increase to values < 100 x 10-. ... 

For the most part, nonpolar chemicals move across the layers of the cell envelope by passive diffusion (Nikaido, 1979 Konings et al., 1981 Sikkema et al., 1995 Bugg et al., 2000). Experimental observations using nonmetabolizable compounds like trichlorobenzene (Ramos et al., 1997 Ramos et al., 1998) and metabolically incompetent mutants with phenanthrene (Bugg et al, 2000) show that passive transfer nonpolar compounds occurs quickly, taking only seconds to minutes. 

The actual blotting process may be accomplished by one of two methods passive (or capillary) transfer and electroblotting. In passive transfer, the membrane is placed in direct contact with the polyacrylamide gel and organized in a sandwich-like arrangement consisting of (from bottom to top) filter paper soaked with transfer buffer, gel, membrane, and more filter paper. The sandwich is compressed by a heavy weight. Buffer passes by capillary ac-... 

Characterization of the cells mediating passive transfer. Cell Immunol 80(l) 198-204 Tchilibon S, Joshi BV, Kim SK, Duong HT, Gao ZG, Jacobson KA (2005) (N)-methanocarba 2, N6-disubstituted adenine nucleosides as highly potent and selective Aj adenosine receptor agonists. J Med Chem 48(6) 1745-1758... 

Mangold, B.L. and Dean, D.A. (1 992) The role of IgG antibodies from irradiated cercaria-immunized rabbits in the passive transfer of immunity to Schistosoma mansoni-infected mice. American journal of Tropical Medicine and Hygiene 47, 821-829. 

Although sheep are not well protected against T. ovis oncospheral invasion by passive transfer of AB in colostrum, mice were protected against T. taeniaeformis by the oral administration of colostral IgA and IgG (Lloyd and Soulsby, 1 978) or serum IgG (Musoke and Williams, 1975) from infected animals. 

Lloyd, S. and Soulsby, E.J. (1978) The role of IgA immunoglobulins in the passive transfer of protection to Taenia taeniaeformis in the mouse. Immunology 34, 939-945. 

The role that the snail haemolymph plays as part of the innate defence arsenal against schistosomes was demonstrated by passive transfer studies where haemolymph from resistant but not susceptible snails was shown to possess a heat-labile factor that specifically affected haemocyte function (Granath and... 

Cranath, W.O. Jr and Yoshino, T.P. (1 984) Schistosoma mansoni passive transfer of resistance by serum in the vector snail, Biomphalaria glabrata. Experimental Parasitology 58, 188-193. 

Leid, R.W. and Williams, J.F. (1 974) The immunological response of the rat to infection with Taenia taeniae-formis. I. Immunoglobulin classes involved in passive transfer of resistance. Immunology 27, 195-208. 

Lloyd, S. and Soulsby, E.J.L. (1976) Passive transfer of immunity to neonatal calves against the metacestodes of Taenia saginata. Veterinary Parasitology 2, 355-362. 

Mitchell, G.F., Goding, J.W. and Rickard, M.D. (1977) Studies on immune responses to larval cestodes in mice. Increased susceptibility of certain mouse strains and hypothymic mice to Taenia taeniaeformis and analysis of passive transfer of resistance with serum. Australian Journal of Experimental Biology and Medical Science 55, 165-1 86. 

AMOUNT OF PASSIVELY TRANSFERRED RABBIT ANTIBODIES ( ELISA end-point )... 

Figure 12. Protective efficiency in mice of passively transferred rabbit antisera against intraperitoneal challenge with S. typhimurium bacteria (U 106 bacteria = 25 LD 0 dose). Percentage ofsurvivors plotted against ELISA titers with S. typhimurium LPS as antigen. Key U, anti whole heat-killed bacterial serum (04, 12 specific) a, anti[GM(A )R],-ITC-BSA serum (04,12) . anti[GM(A )R]2-ITC-BSA serum (04,12) and o, anti AM-ITC-BSA serum (04). (Reproduced with permission from Ref. 65. Copyright 1981, Infect. Immun.j...

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