Parathyroid hormone assay

Andress DL, Endres DB, Maloney NA. Comparison of parathyroid hormone assays with bone histomor-phometry in renal osteodystrophy. J Clin Endocrinol Metab 1986 63 1163-9. 

Malluche HH, Mawad H, Truba D, Monier-Faugere MC. Parathyroid hormone assays—evolution and revolutions in the care of dialysis patients. Chn Nephrol 2003 59 313-8. 

Jara, A., J. Bover, J. Lavigne, and A. Felsenfeld. 1994. Comparison of two parathyroid hormone assays for the rat The new immunoradiometric and the older competitive binding assay. Journal of Bone and Mineral Research 1 1629-1633. 

Terry, A. H., J. Orrock, and A. W. MeiUce. 2003. Comparison of two third-generation parathyroid hormone assays. Clinical Chemistry 49 336-337. 

Table 6.4 Comparison of three parathyroid hormone (PTH) commercial assay kits.

Potts JT, Jr., Bringhurst FR, Gardella TJ, et al. Parathyroid hormone Physiology, chemistry, biosynthesis, secretion, metabolism, and mode of action. In Degroot LJ (Ed.), Endocrinology. W.B. Saunders, Philadelphia, PA (1996) 920-965. Nussbaum SR, Zahradnik RJ, Lavigne JR, et al. Highly sensitive two-site immuno-radiometric assay of parathyrin and its clinical utility in evaluation patients with hypercalcemia. Clin. Chem. (1987) 33 1364-1367. 

Brown RC, Aston JP,Weeks I, et al. Circulation intact parathyroid hormone measured by a two-site immunochemilumino-tetric assay. /. Clin. Endocrinol. Metab. (1987) 65 407-414. 

A non-(l-84) circulating parathyroid hormone (PTH) fragment interferes significantly with intact PTH commercial assay measurements in uremic samples. Clin. Chem. (1998) 44 805-809. 

P2. Pandian, M. R., Morgan, C. H., Carlton, E., and Serge, G. V., Modified immunoradiometric assay of parathyroid hormone-related protein Clinical application in the differential diagnosis of hypercalcemia. Clin. Chem. 38, 282-288 (1992). 

It is now common practice in our laboratory to assay parathyroid hormone by a fully automated system in batches of 60 samples with appropriate controls at intervals of 20 samples. Patient samples are input at the appropriate dilution, this process taking approximately 1 hr. On the reagent addition unit, labeled antibody is added, as well as plasma to standards and buffer to plasma samples. Reagent addition takes 10-15 min. After incubation at 4 for 72 hr, the assay tubes are allowed to adjust to room temperature (the Kemtek 3000 does not have means of refrigeration), and immunoadsorbent is added, with a 10 sec delay between each addition. When addition is complete (30-40 min), filtration is commenced with a cycle that takes 50 sec for its five simultaneous transfers plus washing. The filters are dried and counted. It should be noted that this procedure differs from the standard immunoradiometric technique in that it is the radioactivity not bound to antigen that is counted. The data therefore resemble those derived from a classical radioimmunoassay and can be processed by the Kemtek computer using a polynomial cubic analysis. ... 

Armitage EK. Parathyrin (parathyroid hormone) metabolism and methods of assay. Clin Chem 1986 32 418-24. 

Brossard J-H, Cloutier M, Roy L, Lepage R, Gascon-Barre M, D Amour P. Accumulation of a non-(l-84) molecular form of parathyroid hormone (PTH) detected by intact PTH assay in renal failure importance in the interpretation of PTH values. J Cfin Endocrinol Metab 1996 81 3923-9. 

Brown RC, Aston JP, St John A, Woodhead JS. Comparison of poly- and monoclonal antibodies as labels in a two-site immunochemduminometric assay for intact parathyroid hormone. J Immunol Methods 1988 109 139-44. 

Brown RC, Aston JP, Weeks I, Woodhead JS. Circulating intact parathyroid hormone measured with a two-site immunochemiluminometric assay. J Clin Endocrinol Metab 1987 65 407-14. 

Carter AB, Howanitz PJ. Intraoperative testing for parathyroid hormone a comprehensive review of the use of the assay and the relevant literature. Arch Pathol Lab Med 2003 127 1424-42. 

Moriniere P> Gueris J> et al. Comparison of intact, midregion, and carboxy terminal assays of parathyroid hormone for the diagnosis of bone disease in hemodialysed patients. J Clin Endocrinol Metab 1991 73 516-24. ... 

Curley IR, Wheeler MH, Aston JP, Brown RC, Weeks I, Woodhead JS. Studies in patients with hyperparathyroidism using a two-site immunochemilumi-nometric assay for circulating intact (1-84) parathyroid hormone. Surgery 1987 102 926-31. 

Davis OK, Hawkins DS, Rubin LP, Posillico JT, Brown EM, Schiff I. Serum parathyroid hormone in pregnant women determined by an immunoradiometric assay for intact PTH. J Clin Endocrinol Metab 1988 67 850-2. 

Fraser WD, Robinson J, Lawton R, Durham B, Gallacher SJ, Boyle IT, et al. Clinical and laboratory studies of a new immunoradiometric assay of parathyroid hormone-related protein. Clin Chem 1993 39 414-19. 

Frolich M, Wahna ST, Paulson C> Papapoulos SE. Immunoradiometric assay for intact human parathyroid hormone characteristics, clinical appHcation and comparison with a radioimmunoassay. Ann Clin Biochem 1990 27 69-72. 

Gao P, Scheibel S, D Amour PD, John MR, Rao SD, Schmidt-Gayk H, et al. Development of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone 1-84 implications for improvement of accurate assessment of parathyroid function. J Bone Miner Res 2001 16 605-14. 

Ikeda K, Ohno H, Hane M, Yokoi H, Okada M, Honma T. Development of a sensitive two-site immunoradiometric assay for parathyroid hormone-related peptide evidence for elevated levels in plasma from patient with adult T-ceU leukemia/iymphoma... 

Newman DJ, Ashby JP. Clinical and laboratory evaluation of a two-site immunoradiometric assay for intact parathyroid hormone. Ann Clin Biochem 1988 25 654-60. 

Silverberg SJ, Gao P, Brown I, LoGerfo P, Cantor TL, Bilezikian JP. Clinical utility of an immunoradiomet-ric assay for parathyroid hormone (1-84) in primary hyperparathyroidism. J Clin Endocrinol Metab 2003 88 4725-30. 

Yamashita H, Gao P, Noguchi S, Cantor T, Uchino S, Watanabe S, et al. Role of cyclase activating parathyroid hormone (1-84 PTH) measurements during parathyroid surgery potential improvement of intraoperative PTH assay. Ann Surg 2002 236 ... 

Parathyroid hormone can be assayed by its phosphaturic effect in mice (D2), its calcemic effect in parathyroidectomized rats (Dl, Mil), its effect on serum calcium in the dog (Ul), and its effect on the excretion of isotopic calcium or phosphate in the urine after the labeling of rats with Ca or P (C8, C9). None of these methods is particularly sensitive, and for this reason little work has been done on the estimation of parathyroid hormone in biological fluids. An attempt has been made to meas-... 

D2. Davies, B. M. A., Gordon, A. H., and Mussett, M. V., A mouse urine phosphate assay for parathyroid hormone with certain applications. J. Physiol. (London) 180, 79-95 (1955). 

Calcitonin acts on receptors in bone osteoclasts with a resulting reduction of bone resorption, and it also acts on the renal tubular reabsorption of phosphate. The phosphaturic effects are accompanied by diuresis and increased excretion of other electrolytes. Calcitonin and parathyroid hormone act as a dual negative feedback mechanism in controlling calcium in intra- and extracellular fluids. The range of calcitonin assays suitable for laboratory animals is limited, but the hormone can be measured by two-site radioimmunometric assays (Moukhtar et al. 2005). 

Hypocalcaemia is associated with hypo- and pseudohypoparathyroidism while hypercalcaemia commonly relates to cirrhosis, adrenal insufficiency, osteoporosis, sarcoidosis, disease atrophy, and hyperparathyroidism. Calcium assays are rather important diagnostic aids especially since a routine screening on total serum calcium of 50 330 patients revealed 55 cases of unsuspected primary hyperparathyroidism [137] and the disease is on the increase in the U.S.A. and Sweden [138]. In primary hyperparathyroidism, the glands become autonomous and secretions of parathyroid hormone (PTH) are no longer controlled by the... 

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