Panax ginseng suspension cultures

Table 3. Effects of initial /C+ concentration on the maximum content, production, productivity (Pv) and yield (against sugar) of ginseng saponin (S) and polysaccharide (P) in suspension cultures of Panax ginseng in shake flasks...

Recently, pilot bioreactors as large as 20 kl have been constructed in the research laboratories of Japan Tobacco and Salt Co. and in those of Nitto Denko Co. Solid culture methods were used in large scale pilot experiments for the production of tobacco cells, and liquid culture methods were used in the production of Panax ginseng cells. An outstanding example of cell suspension culture in a pilot scale bioreactor (750 1) was the production of shikonins by Mitui Petrochemical Industries. In all these examples, various technologies have been used to improve the productivity of the metabolites. 

Cultured plant cells may also serve as sources of various naphthoquinones and benzoquinones, whether or not they are present in the whole plants. For example, suspension cultures of Panax ginseng C. A. Meyer were treated with either an elicitor preparation from the phytopathogenic Botrytis cinerea or a yeast elicitor preparation, and the accumulation of a new compound (2,5-dimethoxy-l,4-benzoquinone), which was not detected in non-elicited cultures, was observed [345], Induction of naphthoquinone formation in Impatients balsamina cell cultures was achieved by using parent plants yielding high levels of 2-methoxy- 1,4-naphthoquinone as initiated explants. The cell cultures were capable of producing two naphthoquinones, lawsone... 

Furuya, T., T. Yoshikawa, Y. Orihara, and H. Oda Saponin Production in Cell Suspension Cultures of Panax Ginseng. Planta Medica 48, 83 (1983). 

Furuya, T. Yoshikawa, T. Orihara, Y. Oda, H. (1983). Saponin production in cell suspension cultures of Panax ginseng. Planta Medica, Vol.48, No.2, (June 1983), pp. 83-87, ISSN 0032-0943... 

Ali MB, Yu KW, Hahn EJ, Pack KY (2006) Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors. Plant Cell Rep 25 613-620... 

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