P53 acetylation

Solomon JM, Pasupuleti R, Xu L, McDonagh T, Curtis R, DiStefano PS, Huber LJ (2006) Inhibition of SIRTl catalytic activity increases p53 acetylation but does not alter cell survival following DNA Damage. Mol cell Biol 26(1) 28-38... 

Warnock LJ, Raines SA, Mee TR, Milner J (2005) Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins. FEBS Journal 272(7) 1669-1675... 

Ionov, Y, Nowak N, Perucho M, Markowitz S, Cowell JK (2004) Manipulation of nonsense mediated decay identifies gene mutations in colon cancer Cells with microsatellite instability. Oncogene 23 639-645 Ito A, Lai CH, Zhao X, Saito S, Hamilton MH, Appella E, Yao TP (2001) p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2. EMBO J 20 1331-1340... 

Kobet E, Zeng X, Zhu Y, Keller D, Lu H (2000) MDM2 inhibits p300-mediated p53 acetylation and activation by forming a ternary complex with the two proteins. Proc Natl Acad Sci USA 97 12547-12552... 

Luo, J., et ah, Acetylation of p53 augments its site-specific DNA binding both in vitro and in vivo. Proc Natl Acad Sci USA, 2004, 101(8), 2259-64. 

Figure 2. Regulation of p53 function by acetylation / deacetylation Under stresses conditions p53 gets phosphorylated, acetylated and consequently gets stabilized. Acetylated p53 has enhanced...

Acetylation of p53, E2F and many other proteins not only activates their transcriptional activities but also half-life (Li et al, 2002 Martlnez-Balbas et al, 2000). Apart from DNA binding, and protein half-life, acetylation also plays pivotal role in protein-protein interactions thereby cellular processes involved. However, the acetylation of pRb regulates the specific interaction with E2F-1 (Markham et al, 2006). E2F activity is negatively regulated by its interaction with pRb, which recruits histone decetylase complexes and probably lead to the deacetylation of E2F. 

Gu W, Roeder RG (1997) Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain. Cell 90(4) 595-606... 

Kramer OH, Baus D, Knauer SK, Stein S, Jager E, Stauber RH, Grez M, Pfitzner E, Heinzel T (2006) Acetylation of Stall modulates NF-kappaB activity. Genes Dev 20(4) 473 85 Kumar BR, Swaminathan V, Banerjee S, Kundu TK (2001) p300-mediated acetylation of human transcriptional coactivator PC4 is inhibited by phosphorylation. J Biol Chem 276(20) 16804-16809 Lim JH, West KL, Rubinstein Y, Bergel M, Postnikov YV, Bustin M (2005) Chromosomal protein HMGNl enhances the acetylation of lysine 14 in histone H3. EMBO J 24(17) 3038-3048 Li M, Luo J, Brooks CL, Gu W (2002) Acetylation of p53 inhibits its ubiquitination by Mdm2. J Biol Chem 277(52) 50607-50611... 

Sakaguchi K, Herrera JE, Saito S, Mild T, Bustin M, Vassilev A, Anderson CW, Appella E (1998) DNA damage activates p53 through a phosphorylation-acetylation cascade. Genes Dev 12( 18) 2831-2841 Shikama N, Chan HM, Krstic-Demonacos M, Smith L, Lee CW, Cairns W, La Thangue NB (2000). Functional interaction between nucleosome assembly proteins and p300/CREB-binding protein family coactivators. Mol Cell Biol 20(23) 8933-8943... 

Bariev NA, Liu L, Chehab NH, Mansfield K, Harris KG, Halazonetis TD, Berger SL (2001) Acetylation of p53 activates transcription through recruitment of coactivators/histone acetyltransferases. Mol Cell 8 1243-1254... 

Legube G, Linares LK, Tyteca S, Caron C, Scheffner M, Chevillard-Briet M, Trouche D (2004) Role of the histone acetyl transferase Tip60 in the p53 pathway. J Biol Chem 279 44825 4833 Linggi BE, Brandt SJ, Sun ZW, Hiebert SW (2005) Translating the histone code into leukemia. J Cell Biochem 96 938-950... 

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