Nonsense-mediated decay

Ionov, Y, Nowak N, Perucho M, Markowitz S, Cowell JK (2004) Manipulation of nonsense mediated decay identifies gene mutations in colon cancer Cells with microsatellite instability. Oncogene 23 639-645 Ito A, Lai CH, Zhao X, Saito S, Hamilton MH, Appella E, Yao TP (2001) p300/CBP-mediated p53 acetylation is commonly induced by p53-activating agents and inhibited by MDM2. EMBO J 20 1331-1340... 

The stem-loop structure in the noncoding 3 region of selenoprotein mRNAs has also been termed a SECTS element in mammals although it has a different overall structure. ° In silica analysis of the human genome sequence, using this consensus SECTS element along with the presence of the characteristic UGA codon within an exon, has led to the discovery of several new selenoproteins, including a selenium-dependent methionine sulfoxide reductase. It has been shown that a specific complex exists for selenoprotein synthesis that shuttles between the nucleus and the cytosol. This possibly protects the preformed complex for nonsense-mediated decay to allow for more efficient selenoprotein synthesis. The specific tRNA needed for selenocysteine... 

Lykke-Andersen J (2004) Making structural sense of nonsense-mediated decay. Nat Struct Mol Biol 11 305-... 

Kerr, T. P., Sewry, C. A., Robb, S. A., and Roberts, R. G. (2001). Long mutant dystrophins and variable phenotypes Evasion of nonsense-mediated decay Hum. Genet. 109, 402-407. 

Hentze, M.W. and Kulozik, A.E. (1999) A perfect message RNA surveillance and nonsense-mediated decay. Cell, 96, 307-310. 

Nrg1tmlFej Targeted mutation 1, Frank E Jones The endogenous locus was modified to contain a stop codon in exon 9 and single loxP sites flanking exons 9,10, and 11. The mutation was designed to specifically target the a-isoform, which encoded by exon 9. RT-PCR analysis showed reduced levels of mRNA, putatively due to nonsense mediated decay... 

Nonsense-Mediated Decay and Other mRNA Surveillance Mechanisms Prevent Translation of Improperly Processed mRNAs... 

Nonsense-mediated decay occurs in the cytoplasm of yeast cells. Remarkably, In mammalian cells, there Is evi-... 

Nonsense-mediated decay and other mRNA surveillance mechanisms prevent the translation of improperly processed mRNAs encoding abnormal proteins that might Interfere with functioning of the corresponding normal proteins. 

Mary Ellen Domeier, Daniel R Morse, Scott W. Knight, Michael Potereiko, Brenda L. Bass and Susan E. Mango, A link between RNA interference and nonsense-mediated decay in Caenorhabditis elegans. Science, 289 (2000), 1928-1930. 

Jones RB, Wang F, Luo Y et al 2001 The nonsense-mediated decay pathway and mutually exclusive expression of alternatively spliced FGFR2IIIb and -IIIc mRNAs. J Biol Chem. 276 4158-4167... 

---