Oral vaccination plant-based vaccine

Streatfleld, S.J. (2006). Mucosal immunization using recombinant plant-based oral vaccines. Methods 38(2) 150-157. 

This chapter first provides a description of immunity in general and then more specifically, immunity in the mucosal immune system. The immune response of both intestinal and respiratory tracts will be described in detail as these are the two most common portals of targeted vaccine development for mucosal immunity. The chapter will cover the basis of mucosal immunity using plant-based oral vaccines. Strategies for increasing mucosal immunity, such as the use of adjuvants, will also be discussed. Finally, the chapter will cover the precliiucal tests and various cliiucal trials that are taking place with respect to production of human and veterinary therapeutic proteins in plants. 

Tacket, C.O. (2009). Plant-based oral vaccines results of human trials. Curr. Top. Micro. Immunol. 332 103-117. 

Investigates the basis of mucosal immunity using plant-based oral vaccines... 

A product or reagent that must be kept cold during transit and storage most often between 4° and 8°C. See Elliott, M.A. and Halbert, G.W., Maintaining the cold chain shipping environment for phase I clinical trial distribution, Int. J. Pharm. 299, 49-54, 2005 Streatfield, S.J., Mucosal immunization using recombinant plant-based oral vaccines. Methods 38, 150-157, 2005. 

The heat-labile toxin B subunit of E. coli (LTB) [398], hepatitis B surface antigen [404], respiratory syncytial virus F protein [258], measles virus hemagglutinin [180], and Norwalk virus capsid protein [240, 405, 406] have each been successfully expressed in plants and delivered orally in animals or humans to determine their im-munoprophylactic activity. The first account of a human clinical trial of oral vaccine based on an E. coli enterotoxin as... 

Arakawa T, Chong DK, Langridge WH. Efficacy of a food plant-based oral cholera toxin B subunit vaccine. Nature Biotechnol., 1998 16(3) 292-297. 

Judge, N.A., Mason, H.S., and O Brien, A.D. (2004). Plant cell-based intimin vaccine given orally to mice primed with intimin reduces times of Escherichia coli 0157 H7 shedding in feces. Infect. Immun. 72(1) 168-175. 

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